Abstract
We have examined expression of the N-myc, c-fos and smg p25A genes in two human neuroblastoma cell lines during their differentiation. The decrease in the N-myc gene expression and the increase in the c-fos gene expression are observed during the differentiation of NB-1 cells into neuronal cells and of GOTO cells into Schwann-type cells. On the other hand, the smg p25A, a ras p21-like small GTP-binding protein, gene expression is increased in NB-1 cells but not in GOTO cells during their differentiation, suggesting that smg p25A is closely associated with the neuronal phenotype of neuroblastoma cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bucladesine / pharmacology
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Cell Differentiation / drug effects
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GTP-Binding Proteins / biosynthesis*
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GTP-Binding Proteins / genetics
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Gene Expression Regulation, Neoplastic / drug effects*
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Humans
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Nerve Growth Factors / pharmacology
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Neuroblastoma / pathology*
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Neurons*
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Proto-Oncogene Proteins / biosynthesis*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-myc / biosynthesis*
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Proto-Oncogene Proteins c-myc / genetics
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Proto-Oncogenes
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RNA, Messenger / biosynthesis
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RNA, Neoplasm / biosynthesis
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Schwann Cells*
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism
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rab3 GTP-Binding Proteins
Substances
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Nerve Growth Factors
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-myc
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RNA, Messenger
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RNA, Neoplasm
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Bucladesine
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GTP-Binding Proteins
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rab3 GTP-Binding Proteins