U-2973, a novel B-cell line established from a patient with a mature B-cell leukemia displaying concurrent t(14;18) and MYC translocation to a non-IG gene partner

Eur J Haematol. 2008 Sep;81(3):218-25. doi: 10.1111/j.1600-0609.2008.01098.x. Epub 2008 May 27.

Abstract

B-cell lymphomas/leukemias with simultaneous t(14;18)(q32;q21) and MYC rearrangements have recently been shown to constitute a separate diagnostic entity, presenting with a rapid clinical course and a very poor prognosis. We describe the establishment of an Epstein-Barr virus negative cell line, designated U-2973, from a male patient with a de novo aggressive B-cell lymphoma/leukemia and very high peripheral blast cell count. Flow cytometry of bone marrow cells and U-2973 displayed a mature B-cell phenotype, and immunostaining showed expression of MYC and BCL2. IG gene rearrangement data were consistent with a lymphoid neoplasm of germinal centre derivation. Cytogenetic studies using conventional G-banding, fluorescent in situ hybridization, spectral karyotyping and single nucleotide polymorphism array demonstrated a complex karyotype with both a t(14;18) and double translocations between MYC and a non-IG gene partner located at chromosome 12p12.1.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Base Sequence
  • Bone Marrow Cells / pathology
  • Cell Line*
  • Chromosome Aberrations
  • Chromosomes, Human, Pair 12 / genetics
  • Chromosomes, Human, Pair 14 / genetics*
  • Chromosomes, Human, Pair 18 / genetics*
  • Cytogenetic Analysis
  • Flow Cytometry / methods
  • Genes, Immunoglobulin / genetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Leukemia, B-Cell / diagnosis
  • Leukemia, B-Cell / genetics*
  • Leukocyte Count
  • Lymphoma, B-Cell / diagnosis
  • Lymphoma, B-Cell / genetics*
  • Male
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis / methods
  • Phenotype
  • Polymorphism, Single Nucleotide / genetics
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Proto-Oncogene Proteins c-myc / genetics*
  • Translocation, Genetic*

Substances

  • Proto-Oncogene Proteins c-bcl-2
  • Proto-Oncogene Proteins c-myc