Abstract
Green tea catechins (GTCs) exert chemopreventive effects in many cancer models. Several studies implicate the DNA synthesis marker minichromosome maintenance protein 7 (MCM7) in prostate cancer progression, growth and invasion; representing a novel therapeutic target. In this study, we investigated the effect of GTCs on MCM7 expression in the transgenic adenocarcinoma mouse prostate model (TRAMP). DNA microarray, immunohistochemistry and western blot analysis showed that GTCs significantly suppressed MCM7 in the TRAMP mice treated with GTCs. Our study indicates that the cellular DNA replication factor MCM7 is involved in prostate cancer (CaP) and MCM7 gene expression was reduced by GTCs. Together, these results suggest a possible role of GTCs in CaP chemoprevention in which MCM7 plays a critical role.
Keywords:
chemoprevention; green tea catechins; minichromosome maintenance protein 7; prostate cancer; transgenic adenocarcinoma mouse prostate model.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism*
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Adenocarcinoma / prevention & control
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Animals
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Biomarkers, Tumor / biosynthesis*
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Biomarkers, Tumor / genetics
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Catechin / pharmacology*
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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DNA Replication / drug effects*
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DNA, Neoplasm / biosynthesis*
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DNA, Neoplasm / genetics
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Expression Regulation, Neoplastic / drug effects
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Male
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Mice
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Mice, Transgenic
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Minichromosome Maintenance Complex Component 7
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Neoplasms, Experimental / metabolism
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Neoplasms, Experimental / prevention & control
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Prostatic Neoplasms / genetics
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Prostatic Neoplasms / metabolism*
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Prostatic Neoplasms / prevention & control
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Tea*
Substances
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Biomarkers, Tumor
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Cell Cycle Proteins
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DNA, Neoplasm
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DNA-Binding Proteins
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Nuclear Proteins
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Tea
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Catechin
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Mcm7 protein, mouse
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Minichromosome Maintenance Complex Component 7