Differential IL-4/Stat6 activities correlate with differential expression of regulatory genes SOCS-1, SHP-1, and PP2A in colon cancer cells

Qin Yuan; Pin Dong Li; Ben Hui Li; Xian Zi Yang; Shuang Bing Xu; Xiao Hong Liu; Fu Xiang Zhou; Wen Jie Zhang

doi:10.1007/s00432-008-0429-8

Differential IL-4/Stat6 activities correlate with differential expression of regulatory genes SOCS-1, SHP-1, and PP2A in colon cancer cells

J Cancer Res Clin Oncol. 2009 Jan;135(1):131-40. doi: 10.1007/s00432-008-0429-8. Epub 2008 Jun 7.

Authors

Qin Yuan¹, Pin Dong Li, Ben Hui Li, Xian Zi Yang, Shuang Bing Xu, Xiao Hong Liu, Fu Xiang Zhou, Wen Jie Zhang

Affiliation

¹ Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, 430030, Wuhan, Hubei, China.

PMID: 18536936
DOI: 10.1007/s00432-008-0429-8

Abstract

Purpose: To investigate potential differences in the expression of Stat6 regulatory genes that may influence IL-4/Stat6 activities (phenotypes) in colon cancer cells.

Methods: RT-PCR method was employed to examine the constitutive mRNA expression of Stat6 negative regulators SOCS-1 and SHP-1, and positive regulator PP2A in colon cancer cell lines HT-29 and Caco-2. Stat6 protein expression and nuclear phosphorylation were detected using Western blotting.

Results: Caco-2 cells carrying inactive Stat6(null) phenotype showed normal constitutive expression of Stat6 but decreased phosphorylation of nuclear Stat6 compared with HT-29 cells carrying active Stat6(high) phenotype. Stat6(null) Caco-2 cells expressed increased levels of mRNA and protein of SOCS-1 and SHP-1, and decreased mRNA expression of PPP2CA and PPP2CB, encoding two critical subunits of PP2A.

Conclusions: Constitutively increased expression of Stat6 negative regulators SOCS-1 and SHP-1, together with decreased expression of positive regulator PP2A, may play a role in forming the inactive Stat6(null) phenotype in colon cancer cells.

MeSH terms

Blotting, Western
Cell Nucleus / metabolism
Colonic Neoplasms / genetics*
Colonic Neoplasms / metabolism
Cytoplasm / metabolism
Humans
Interleukin-4 / genetics*
Interleukin-4 / metabolism
Phenotype
Phosphorylation
Protein Phosphatase 2 / genetics*
Protein Phosphatase 2 / metabolism
Protein Tyrosine Phosphatase, Non-Receptor Type 6 / genetics*
Protein Tyrosine Phosphatase, Non-Receptor Type 6 / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
STAT6 Transcription Factor / genetics*
STAT6 Transcription Factor / metabolism
Suppressor of Cytokine Signaling 1 Protein
Suppressor of Cytokine Signaling Proteins / genetics*
Suppressor of Cytokine Signaling Proteins / metabolism
Tumor Cells, Cultured

Substances

IL4 protein, human
RNA, Messenger
SOCS1 protein, human
STAT6 Transcription Factor
STAT6 protein, human
Suppressor of Cytokine Signaling 1 Protein
Suppressor of Cytokine Signaling Proteins
Interleukin-4
PPP2CA protein, human
PPP2CB protein, human
Protein Phosphatase 2
PTPN6 protein, human
Protein Tyrosine Phosphatase, Non-Receptor Type 6