Expression of multidrug resistance genes in normal and cancer stem cells

Cancer Invest. 2008 Jun;26(5):535-42. doi: 10.1080/07357900801904140.

Abstract

CD133 (stem marker) and drug resistant gene (mdr1 and bcl-2) expression were detected in human glioblastoma and normal brain tissues. Glioma tissues contained higher ratio of CD133+ cells compared to normal tissues ( approximately 10 fold increase). The chemosensitivity assay showed that GOS-3 and NHA CD133+ possess greater resistance towards drugs compared to CD133- fractions. This study revealed for the first time that: a) serum deprivation enriched CD133 expression and demonstrated a direct co-expression between CD133 and drug resistant in GOS-3 cells and b) higher expression of CD133 and drug resistance were found in glioblastoma tissues in comparison to normal brain tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AC133 Antigen
  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics*
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
  • Antigens, CD / genetics
  • Antigens, CD / metabolism*
  • Antineoplastic Agents / pharmacology
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / immunology
  • Brain Neoplasms / metabolism
  • Brain Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Survival
  • Culture Media, Serum-Free
  • Dose-Response Relationship, Drug
  • Drug Resistance, Multiple / genetics*
  • Drug Resistance, Neoplasm / genetics*
  • Flow Cytometry
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Glioblastoma / genetics*
  • Glioblastoma / immunology
  • Glioblastoma / metabolism
  • Glioblastoma / pathology
  • Glycoproteins / genetics
  • Glycoproteins / metabolism*
  • Humans
  • Immunomagnetic Separation
  • Inhibitory Concentration 50
  • Intermediate Filament Proteins / metabolism
  • Neoplastic Stem Cells / drug effects
  • Neoplastic Stem Cells / immunology
  • Neoplastic Stem Cells / metabolism*
  • Nerve Tissue Proteins / metabolism
  • Nestin
  • Peptides / genetics
  • Peptides / metabolism*
  • Proto-Oncogene Proteins c-bcl-2 / genetics*
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Up-Regulation

Substances

  • ABCB1 protein, human
  • AC133 Antigen
  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Antigens, CD
  • Antineoplastic Agents
  • Culture Media, Serum-Free
  • Glycoproteins
  • Intermediate Filament Proteins
  • NES protein, human
  • Nerve Tissue Proteins
  • Nestin
  • PROM1 protein, human
  • Peptides
  • Proto-Oncogene Proteins c-bcl-2