Abstract
CD133 (stem marker) and drug resistant gene (mdr1 and bcl-2) expression were detected in human glioblastoma and normal brain tissues. Glioma tissues contained higher ratio of CD133+ cells compared to normal tissues ( approximately 10 fold increase). The chemosensitivity assay showed that GOS-3 and NHA CD133+ possess greater resistance towards drugs compared to CD133- fractions. This study revealed for the first time that: a) serum deprivation enriched CD133 expression and demonstrated a direct co-expression between CD133 and drug resistant in GOS-3 cells and b) higher expression of CD133 and drug resistance were found in glioblastoma tissues in comparison to normal brain tissues.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AC133 Antigen
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics*
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
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Antigens, CD / genetics
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Antigens, CD / metabolism*
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Antineoplastic Agents / pharmacology
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Brain Neoplasms / genetics*
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Brain Neoplasms / immunology
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Brain Neoplasms / metabolism
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Brain Neoplasms / pathology
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Cell Line, Tumor
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Cell Proliferation
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Cell Survival
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Culture Media, Serum-Free
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Dose-Response Relationship, Drug
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Drug Resistance, Multiple / genetics*
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Drug Resistance, Neoplasm / genetics*
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Flow Cytometry
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic
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Glioblastoma / genetics*
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Glioblastoma / immunology
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Glioblastoma / metabolism
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Glioblastoma / pathology
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Glycoproteins / genetics
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Glycoproteins / metabolism*
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Humans
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Immunomagnetic Separation
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Inhibitory Concentration 50
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Intermediate Filament Proteins / metabolism
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Neoplastic Stem Cells / drug effects
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Neoplastic Stem Cells / immunology
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Neoplastic Stem Cells / metabolism*
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Nerve Tissue Proteins / metabolism
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Nestin
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Peptides / genetics
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Peptides / metabolism*
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Proto-Oncogene Proteins c-bcl-2 / genetics*
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Up-Regulation
Substances
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ABCB1 protein, human
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AC133 Antigen
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antigens, CD
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Antineoplastic Agents
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Culture Media, Serum-Free
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Glycoproteins
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Intermediate Filament Proteins
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NES protein, human
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Nerve Tissue Proteins
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Nestin
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PROM1 protein, human
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Peptides
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Proto-Oncogene Proteins c-bcl-2