Objective: Objective Based on the distribution of genetic polymorphisms of GSTM1 ,GSTT1 and CYP1A1 genotypes in mothers and neonates with preterm delivery and health controls, to explore the relationship between these gene polymorphisms and susceptibility to preterm delivery.
Methods: we investigated mothers and neonates with preterm delivery and health controls were investigated by case-control method. The frequencies of GSTM1, GSTr1 and CYP1A1 genotypes were detected by PCR and RFLP-PCR techniques.
Results: The difference of variation frequencies of GSTM1, GSTT1 and CYP1A1 (Msp I polymorphism) genotypes between cases (mothers and neonates respectively) and their health controls were not statistically significant (P > 0.05). The difference of variation frequencies of GSTM1 and GSTT1 combined between mother cases group and control groups were not statistically significant (P > 0.05). The neonates was same as mother groups. However, the differences of CYP1A1 heterozygous mutation and homozygous mutation gene combined with the null genotypes among GSTT1 between mother groups with preterm delivery and health controls were statistically significant (chi2 = 4.683, P < 0.05, OR = 2.440).
Conclusion: CYP1A1 heterozygous mutation and homozygous mutation gene combined with GSTT1 null genotypes in mothers could increase the risk of preterm delivery.