Loss of heterozygosity of the APC gene in oral squamous cell carcinoma

Elena R C Rivero; Martinho C R Horta; Eliete N Silva Guerra; Alberto R Ferraz; Fábio D Nunes

doi:10.1016/j.prp.2008.05.003

Loss of heterozygosity of the APC gene in oral squamous cell carcinoma

Pathol Res Pract. 2008;204(11):793-7. doi: 10.1016/j.prp.2008.05.003. Epub 2008 Jul 9.

Authors

Elena R C Rivero¹, Martinho C R Horta, Eliete N Silva Guerra, Alberto R Ferraz, Fábio D Nunes

Affiliation

¹ Department of Pathology, Health Science Center, Federal University of Santa Catarina, CEP 88040-370, Florianópolis, SC, Brazil. riet@ccs.ufsc.br

PMID: 18617334
DOI: 10.1016/j.prp.2008.05.003

Abstract

The aim of this study was to investigate loss of heterozygosity (LOH) of the APC tumor suppressor gene loci, using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) in 40 cases of oral squamous cell carcinoma (OSCC). Observed informativity was 72.5% for APC exon 11 and 82.5% for APC exon 15. LOH at APC exon 11 was observed in 2 (6.9%) of 29 informative cases, and no LOH was observed for APC exon 15. Our results suggest that inactivation of the APC gene plays a minor role in the carcinogenesis of OSCC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma, Squamous Cell / genetics*
Genes, APC*
Humans
Loss of Heterozygosity*
Mouth Neoplasms / genetics*
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length