Abstract
Oral squamous cell carcinoma (OSCC) is the 11th most common cancer worldwide, and is associated with a high death rate. At present, there are no suitable markers for detecting and/or monitoring OSCC in body fluids/tissues. Here, we used 1D SDS-PAGE and MALDI-TOF MS to systematically analyze the secretomes of two OSCC cell lines (OEC-M1 and SCC4). The putative OSCC-related proteins identified in this analysis included the Mac-2 binding protein (Mac-2 BP), which was further found to be overexpressed in OSCC specimens and significantly elevated in the sera of OSCC patients compared to healthy controls. Finally, RNA interference-based knock-down of Mac-2 BP expression in OSCC cells revealed for the first time that Mac-2 BP is involved in regulating growth and motility of OSCC cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Antigens, Neoplasm / analysis*
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Antigens, Neoplasm / blood
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Base Sequence
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Biomarkers, Tumor / analysis*
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Biomarkers, Tumor / blood
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Carcinoma, Squamous Cell / diagnosis
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Carcinoma, Squamous Cell / metabolism*
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Carcinoma, Squamous Cell / pathology
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Cell Cycle
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Cell Division*
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Cell Movement*
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Culture Media, Conditioned
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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Female
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Humans
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Immunohistochemistry
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Male
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Membrane Glycoproteins / analysis*
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Membrane Glycoproteins / blood
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Middle Aged
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Mouth Neoplasms / diagnosis
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Mouth Neoplasms / metabolism*
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Mouth Neoplasms / pathology
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Proteome*
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RNA Interference
Substances
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Antigens, Neoplasm
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Biomarkers, Tumor
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Culture Media, Conditioned
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Membrane Glycoproteins
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Proteome
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TAA90K protein, human