Cellular protein TIA-1 regulates the expression of HBV surface antigen by binding the HBV posttranscriptional regulatory element

Intervirology. 2008;51(3):203-9. doi: 10.1159/000151632. Epub 2008 Aug 28.

Abstract

Objective: To identify T-cell intracellular antigen 1 (TIA-1) interacting with the posttranscriptional regulatory element (PRE) of hepatitis B virus (HBV), and reveal TIA-1 function on HBV gene expression.

Methods: In order to demonstrate TIA-1 binding to PRE, electrophoretic mobility shift and RNA and protein pull-down assays were used. The pDM138-PRE reporter system, HepG2.2.15 cell line and HBs-PRE transient expression cells were adopted to identify TIA-1 function in the HBV life cycle.

Results: TIA-1 could directly bind to PRE RNA. The functional binding region of TIA-1 was identified. Functional analysis indicated that TIA-1 could dose-dependently inhibit PRE function in pDM138-PRE system. Down-expression of HBs antigen induced by TIA-1 was detected both in the HepG2.2.15 cell line and in HBs-PRE transient expression cells.

Conclusion: These data demonstrated that TIA-1 inhibits HBs expression by interacting with PRE.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cell Line
  • Electrophoretic Mobility Shift Assay
  • Hepatitis B Surface Antigens / biosynthesis*
  • Hepatitis B virus / physiology*
  • Humans
  • Poly(A)-Binding Proteins / metabolism*
  • Protein Binding
  • RNA Processing, Post-Transcriptional
  • RNA, Viral / metabolism
  • T-Cell Intracellular Antigen-1

Substances

  • Hepatitis B Surface Antigens
  • Poly(A)-Binding Proteins
  • RNA, Viral
  • T-Cell Intracellular Antigen-1
  • TIA1 protein, human