Abstract
The pre-replication complex (pre-RC), including the core hexameric MCM2-7 complex, ensures that the eukaryotic genome is replicated only once per cell division cycle. In this study, we identified a rice minichromosome maintenance (MCM) homologue (OsMCM2) that functionally complemented fission yeast MCM2 (CDC19) mutants. We found OsMCM2 transcript expression in roots, leaves, and seeds, although expression levels differed slightly among the organs. Likewise, the OsMCM2 protein was ubiquitously expressed, but it was downregulated when nutritients were limiting, indicating that MCM2 expression (and therefore cell cycle progression) requires adequate nutrition. Yeast two-hybrid and GST pull-down assays demonstrated that OsMCM2 interacted with the COP9 signalosome 5 (CSN5). Taken as a whole, our results indicated that OsMCM2 functions as a subunit of the rice MCM complex and interacts with CSN5 during developmental regulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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COP9 Signalosome Complex
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism
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DNA Primers / genetics
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DNA Replication / genetics*
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DNA, Plant / biosynthesis
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DNA, Plant / genetics
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Gene Expression
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Genes, Plant
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Genetic Complementation Test
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Molecular Sequence Data
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Multiprotein Complexes / genetics
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Multiprotein Complexes / metabolism
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Oryza / genetics*
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Oryza / growth & development
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Oryza / metabolism*
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Peptide Hydrolases / genetics
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Peptide Hydrolases / metabolism
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Phylogeny
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Plant Proteins / metabolism*
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Schizosaccharomyces pombe Proteins / genetics
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Schizosaccharomyces pombe Proteins / metabolism
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Species Specificity
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Tissue Distribution
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Two-Hybrid System Techniques
Substances
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Cell Cycle Proteins
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DNA Primers
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DNA, Plant
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Multiprotein Complexes
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Plant Proteins
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Schizosaccharomyces pombe Proteins
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Peptide Hydrolases
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COP9 Signalosome Complex
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mcm2 protein, S pombe