Abstract
Aim:
To investigate the role of livin and vascular endothelial growth factor (VEGF) in human esophageal carcinoma, and analyze its relationship to clinical stages.
Methods:
Expression of livin in fresh esophageal cancer tissues was detected by immunohistochemistry (IHC), Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR), and VEGF by Western blotting and RT-PCR. All statistical analyses were performed by SPSS version 11.0.
Results:
Livin positivity was also significantly correlated with tumor stages, increasing with tumor progression. Expression of livin and VEGF increased with the process of esophageal carcinoma. In the fourth clinical stage, expression of livin and VEGF was the most significant. Expression of Livin was positively correlated with VEGF.
Conclusion:
Over-expression of livin and VEGF contributes to the pathogenesis of esophageal carcinoma.
MeSH terms
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Adaptor Proteins, Signal Transducing / analysis*
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Adaptor Proteins, Signal Transducing / genetics
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Adult
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Aged
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Aged, 80 and over
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Blotting, Western
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Carcinoma / chemistry*
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Carcinoma / genetics
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Carcinoma / pathology
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Esophageal Neoplasms / chemistry*
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Esophageal Neoplasms / genetics
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Esophageal Neoplasms / pathology
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Immunohistochemistry
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Inhibitor of Apoptosis Proteins / analysis*
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Inhibitor of Apoptosis Proteins / genetics
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Male
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Middle Aged
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Neoplasm Proteins / analysis*
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Neoplasm Proteins / genetics
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Neoplasm Staging
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RNA, Messenger / analysis
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Reverse Transcriptase Polymerase Chain Reaction
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Up-Regulation
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Vascular Endothelial Growth Factor A / analysis*
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Vascular Endothelial Growth Factor A / genetics
Substances
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Adaptor Proteins, Signal Transducing
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BIRC7 protein, human
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Inhibitor of Apoptosis Proteins
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Neoplasm Proteins
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RNA, Messenger
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VEGFA protein, human
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Vascular Endothelial Growth Factor A