Abstract
The effect of ganoderic acid Me (GA-Me), which was purified from the fermentation mycelia of the traditional Chinese medicinal mushroom Ganoderma lucidum as reported (Tang W, Gu TY, Zhong JJ. Biochem Eng J. 2006;32:205-210), on anti-invasion was investigated. Wound healing assay indicated that GA-Me inhibited cell migration of 95-D, a human highly metastatic lung tumor cell line, in dose- and time-dependent manners. Results of cell aggregation and adhesion assays showed that GA-Me promoted cell homotypic aggregation and inhibited cell adherence to extracellular matrix (ECM). In addition, GA-Me suppressed matrix metalloproteinases 2/9 (MMP2/9) gene expressions at both mRNA and protein levels in 95-D cells according to qRT-PCR and Western blotting, respectively. The results demonstrated that GA-Me effectively inhibited tumor invasion, and it might act as a new MMP2/9 inhibitor for anti-metastasis treatment of carcinoma cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / pharmacology*
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Cell Adhesion / drug effects
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Cell Aggregation / drug effects
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Cell Line, Tumor
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Cell Movement / drug effects
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Dose-Response Relationship, Drug
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Down-Regulation
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Gene Expression Regulation, Enzymologic / drug effects*
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Gene Expression Regulation, Neoplastic / drug effects*
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Humans
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Lung Neoplasms / enzymology*
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Lung Neoplasms / genetics
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Lung Neoplasms / pathology
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Matrix Metalloproteinase 2 / genetics
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Matrix Metalloproteinase 2 / metabolism
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Matrix Metalloproteinase 9 / genetics
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Matrix Metalloproteinase 9 / metabolism
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Matrix Metalloproteinase Inhibitors*
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Neoplasm Invasiveness
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Protease Inhibitors / pharmacology*
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RNA, Messenger / metabolism
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Time Factors
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Triterpenes / pharmacology*
Substances
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Antineoplastic Agents, Phytogenic
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Matrix Metalloproteinase Inhibitors
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Protease Inhibitors
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RNA, Messenger
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Triterpenes
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ganoderic acid Me
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MMP2 protein, human
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9