Abstract
Apolipoprotein AI (apoAI) is a lipid-binding protein that participates in the transport of cholesterol and other lipids in the plasma. A complementary DNA clone for a protein that bound to regulatory elements of the apoAI gene was isolated. This protein, designated apoAI regulatory protein-1 (ARP-1), is a novel member of the steroid hormone receptor superfamily. ARP-1 bound to DNA as a dimer, and its dimerization domain was localized to the COOH-terminal region. ARP-1 also bound to a thyroid hormone-responsive element and to regulatory regions of the apoB, apoCIII, insulin, and ovalbumin genes. In cotransfection experiments, ARP-1 downregulated the apoAI gene. The involvement of ARP-1 in the regulation of apoAI gene expression suggests that it may participate in lipid metabolism and cholesterol homeostasis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Apolipoprotein A-I
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Apolipoproteins A / genetics*
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Base Sequence
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COUP Transcription Factor II
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COUP Transcription Factors
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Cloning, Molecular
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DNA / metabolism
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DNA-Binding Proteins / metabolism*
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Gene Expression Regulation
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Humans
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Lipoproteins, HDL / genetics*
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Molecular Sequence Data
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Oligonucleotide Probes
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Receptors, Steroid / metabolism*
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Regulatory Sequences, Nucleic Acid
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Transcription Factors*
Substances
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Apolipoprotein A-I
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Apolipoproteins A
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COUP Transcription Factor II
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COUP Transcription Factors
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DNA-Binding Proteins
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Lipoproteins, HDL
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NR2F2 protein, human
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Oligonucleotide Probes
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Receptors, Steroid
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Transcription Factors
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DNA
Associated data
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GENBANK/M55283
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GENBANK/M64497
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GENBANK/S71725
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GENBANK/X53015
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GENBANK/X53016
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GENBANK/X53017
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GENBANK/X53018
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GENBANK/X53019
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GENBANK/X53020
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GENBANK/X53021