Comparison of SYBR Green I and TaqMan real-time PCR formats for the analysis of her2 gene dose in human breast tumors

Bull Exp Biol Med. 2008 Feb;145(2):240-4. doi: 10.1007/s10517-008-0060-3.

Abstract

We compared two technologies of real-time PCR (with the use of fluorescent SYBR Green I dye and specific TaqMan probe) for quantification of the dose of her2 gene in breast tumors. The maximum increase in the gene dose in TaqMan and SYBR Green I analyses was 10- and 5-fold, respectively. In was found that TaqMan and SYBR Green I technologies allow detection of the matrix in amounts corresponding to 1-100 and 2.5-40.0 ng genomic DNA, respectively. Tenfold increase in the gene dose leads to incorrect evaluation of multiplication ratio in the SYBR Green I analysis. These results suggest that TaqMan technology is more preferable for correct evaluation of her2 gene dose.

Publication types

  • Evaluation Study

MeSH terms

  • Benzothiazoles
  • Breast Neoplasms / genetics*
  • Diamines
  • Female
  • Fluorescent Dyes / metabolism*
  • Gene Dosage*
  • Humans
  • Organic Chemicals / metabolism*
  • Polymerase Chain Reaction* / instrumentation
  • Polymerase Chain Reaction* / methods
  • Quinolines
  • Receptor, ErbB-2 / genetics*

Substances

  • Benzothiazoles
  • Diamines
  • Fluorescent Dyes
  • Organic Chemicals
  • Quinolines
  • SYBR Green I
  • ERBB2 protein, human
  • Receptor, ErbB-2