Abstract
Isocitrate dehydrogenase 2 (Icd-2, Rv0066c) from Mycobacterium tuberculosis was cloned and heterologously expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and crystallized. A complete data set has been collected and reduced to 3.25 A resolution in space group C2. Preliminary diffraction data analysis suggests a complex packing with at least six molecules in the asymmetric unit.
MeSH terms
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Cloning, Molecular
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Crystallization
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Crystallography, X-Ray
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Isocitrate Dehydrogenase / chemistry*
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Isocitrate Dehydrogenase / genetics
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Isocitrate Dehydrogenase / isolation & purification
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Mycobacterium tuberculosis / enzymology*
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Mycobacterium tuberculosis / metabolism
Substances
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Bacterial Proteins
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Isocitrate Dehydrogenase