Abstract
Multidrug resistant (MDR) tumor cells over-expressing P-glycoprotein exhibit variation in invasive behavior. To investigate the mechanisms, we analyzed the expression of CD147. The results showed that CD147 expression was increased in HepG2/Adr cells, as compared to HepG2 cells. The MDR cells produced more MMP11 and MDR1, which promoted HepG2/Adr cells invasion and increased resistance to chemotherapeutic drugs. On the other hand, CD147 silencing in HepG2/Adr cells by RNAi led to the opposite effect. Treatment of tumor cells with U-0126, an inhibitor of MAPK/Erk, also down-regulated MMP11 and MDR1 expression. Thus, CD147 may functionally mediate tumor cells invasion and MDR.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
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Antineoplastic Agents / therapeutic use
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Basigin / genetics*
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Carcinoma, Hepatocellular / drug therapy
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Carcinoma, Hepatocellular / enzymology
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Carcinoma, Hepatocellular / genetics*
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Carcinoma, Hepatocellular / immunology
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Carcinoma, Hepatocellular / pathology
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Cell Line, Tumor
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DNA Primers
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Drug Resistance, Multiple
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Humans
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Liver Neoplasms / drug therapy
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Liver Neoplasms / enzymology
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Liver Neoplasms / genetics*
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Liver Neoplasms / immunology
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Liver Neoplasms / pathology
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Matrix Metalloproteinase 11 / metabolism
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Neoplasm Invasiveness
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RNA Interference
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RNA, Neoplasm / genetics
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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ABCB1 protein, human
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antineoplastic Agents
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DNA Primers
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RNA, Neoplasm
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Basigin
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Matrix Metalloproteinase 11