Regular physical activity exerts beneficial effects for mental and physical health, but an intense exercise can cause oxidative stress (OS) in dopaminergic regions and intensify the harmful effects of reserpine. Reserpine-induced neurotoxicity can be accessed by behavioral and biochemical evaluations. The objective of this study was to examine the effect of a gradual intensifying exercise program on an animal model of oxidative stress. Male rats were submitted to swimming sessions (1 h/day, for eleven weeks), and they were loaded gradually during the adaptation period (two weeks) with a weight corresponding to 1-7% of their body weight tied to their back. After the last training, the animals were treated with two doses of vehicle or reserpine (1 mg/kg-sc), an agent that induces orofacial dyskinesia. After behavioral evaluations, the striatum was dissected for enzymatic and biochemical assays. Development of cardiac hypertrophy demonstrated the effectiveness of the physical training. The gradual intense exercise and reserpine increased lipid peroxidation and striatal catalase activity. The results confirm the importance of catalase activity in orofacial dyskinesia which can be related to lipid peroxidation in striatal dopaminergic brain tissue. These results indicate that intense exercise can have some deleterious effect on striatal dopaminergic system.