Abstract
BCL11A on chromosome 2p16 was recently shown to be a major quantitative trait locus for Hb F level and F-cell number in several populations with or without beta-hemoglobinopathy. We now show that BCL11A isoforms are expressed in K562 cells. Butyrate induction of HBG globin production in K562 is associated with reduced BCL11A. Conversely, augmented expression of BCL11A in K562 cells through transfection of BCL11A expression vector results in more than 50% reduction of HBG promoter transcription activity. This transcription repression can be abrogated by sodium butyrate. BCL11A binds to GGCCGG motif in nucleotide -56 to -51 on the HBG proximal promoter. Together, these data are consistent with BCL11A being able to bind to a core motif in the HBG proximal promoter, recruit and interact with partners to form a repression complex, leading to deacetylation of histones and down-regulation of the HBG transcription.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Binding Sites
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Butyrates / pharmacology
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Carrier Proteins / biosynthesis
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Carrier Proteins / genetics
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Carrier Proteins / physiology*
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Cell Differentiation / drug effects
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Gene Dosage
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Humans
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Hydroxamic Acids / pharmacology
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K562 Cells / drug effects
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K562 Cells / metabolism
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Mutagenesis, Site-Directed
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Nuclear Proteins / biosynthesis
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Nuclear Proteins / genetics
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Nuclear Proteins / physiology*
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Polymorphism, Single Nucleotide
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Promoter Regions, Genetic / genetics
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RNA, Messenger / biosynthesis
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Recombinant Fusion Proteins / physiology
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Repressor Proteins / genetics
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Repressor Proteins / physiology*
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Transcription, Genetic / drug effects
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gamma-Globins / biosynthesis*
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gamma-Globins / genetics
Substances
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BCL11A protein, human
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Butyrates
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Carrier Proteins
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Hydroxamic Acids
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Nuclear Proteins
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RNA, Messenger
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Recombinant Fusion Proteins
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Repressor Proteins
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gamma-Globins
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trichostatin A