Abstract
Retinoids triggers differentiation of acute promyelocytic leukemia (APL) blasts by transcriptional regulation of myeloid regulatory genes. Using a microarray approach, we have identified a novel retinoid-responsive gene (CXXC5) encoding a nuclear factor, retinoid-inducible nuclear factor (RINF), that contains a CXXC-type zinc-finger motif. RINF expression correlates with retinoid-induced differentiation of leukemic cells and with cytokine-induced myelopoiesis of normal CD34(+) progenitors. Furthermore, short hairpin RNA (shRNA) interference suggests for this gene a regulatory function in both normal and tumoral myelopoiesis. Interestingly, RINF localizes to 5q31.3, a small region often deleted in myeloid leukemia (acute myeloid leukemia [AML]/myelodysplasia [MDS]) and suspected to harbor one or several tumor suppressor gene.
Publication types
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Amino Acid Sequence
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Carrier Proteins / genetics
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Carrier Proteins / physiology*
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DNA-Binding Proteins
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Gene Expression Profiling
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Gene Expression Regulation / drug effects
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Granulocyte Precursor Cells / drug effects
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Granulocyte Precursor Cells / physiology
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HL-60 Cells
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Hematologic Neoplasms / genetics*
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Humans
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / physiology*
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K562 Cells
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Models, Biological
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Molecular Sequence Data
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Myelopoiesis / drug effects
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Myelopoiesis / genetics*
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Oligonucleotide Array Sequence Analysis
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Sequence Homology, Amino Acid
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Transcription Factors
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Tretinoin / pharmacology
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Tumor Cells, Cultured
Substances
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CXXC5 protein, human
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Carrier Proteins
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DNA-Binding Proteins
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Intracellular Signaling Peptides and Proteins
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Transcription Factors
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Tretinoin