Abstract
Here we investigated the roles of P28GANK in multidrug resistance of gastric cancer cells and the possible mechanisms. We constructed the siRNA vector of P28GANK and transfected it into human vincristine-resistant gastric adenocarcinoma cell line SGC7901/VCR. Down-regulation of P28GANK could enhance the sensitivity of SGC7901/VCR cells towards anticancer drugs and could decrease the capacity of cells to efflux adriamycin. P28GANK could down-regulate the expression of P-gp, but not affect MRP or GST. In vivo experiment also confirmed our above results. Further study of the biological functions of P28GANK might be helpful for understanding the mechanisms of MDR in gastric cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
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Animals
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Apoptosis
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Cell Line, Tumor
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Doxorubicin / pharmacokinetics
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Drug Resistance, Multiple
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Drug Resistance, Neoplasm
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Glutathione Transferase / metabolism
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Humans
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Mice
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Neoplasm Transplantation
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Proteasome Endopeptidase Complex / genetics
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Proteasome Endopeptidase Complex / physiology*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / physiology*
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Stomach Neoplasms / drug therapy*
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Stomach Neoplasms / genetics
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Transplantation, Heterologous
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Verapamil / pharmacology
Substances
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ABCB1 protein, human
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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PSMD10 protein, human
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Proto-Oncogene Proteins
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Doxorubicin
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Verapamil
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Glutathione Transferase
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Proteasome Endopeptidase Complex