Abstract
Accumulating data suggested that CXCR4/SDF-1 pathway may play an important role in the metastasis of tumor. We previously demonstrated that CpG ODN could enhance the metastasis of human lung cancer cell via TLR9. Here we further evaluated the possible role of CXCR4/SDF-1 pathway in the enhanced metastasis of human lung cancer 95D cells induced by CpG ODN. Our data showed down-regulation of CXCR4 expression using siRNA against CXCR4 could significantly reduce the enhanced metastasis of 95D cells induced by CpG ODN both in vitro and in vivo. These results suggested that TLR9 agonist might promote the metastasis of human lung cancer cells via CXCR4/SDF-1 pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Movement
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Chemokine CXCL12 / genetics
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Chemokine CXCL12 / metabolism*
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Down-Regulation
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Humans
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology*
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Mice
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Mice, Nude
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Neoplasm Metastasis
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Oligodeoxyribonucleotides / pharmacology*
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Phosphorylation
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Proto-Oncogene Proteins c-akt / metabolism
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RNA, Small Interfering / genetics
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Receptors, CXCR4 / genetics
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Receptors, CXCR4 / metabolism*
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Toll-Like Receptor 9 / agonists*
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Xenograft Model Antitumor Assays
Substances
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CXCL12 protein, human
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CXCR4 protein, human
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Chemokine CXCL12
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CpG ODN 2216
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Oligodeoxyribonucleotides
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RNA, Small Interfering
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Receptors, CXCR4
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TLR9 protein, human
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Toll-Like Receptor 9
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Proto-Oncogene Proteins c-akt