Abstract
We examined the anti-angiogenic effects of Ganoderma tsugae methanol extract (GTME) on human epidermoid carcinoma A-431 cells. Our data indicate that GTME inhibits the expression of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) in vitro and in vivo, and also inhibits the capillary tube formation of human umbilical vein endothelial cells (HUVECs). We also show that the suppression of VEGF expression by GTME can be restored by treatment with EGF. These results suggest that GTME inhibits VEGF expression via the suppression of EGFR expression, resulting in the downregulation of VEGF secretion from epidermoid carcinoma A-431 cells. These findings reveal a novel role for G. tsugae in inhibiting EGFR and VEGF expression, which are important for tumor angiogenesis and growth. Thus, GTME may provide a potential therapeutic approach for anti-tumor treatment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Angiogenesis Inhibitors / pharmacology*
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Angiogenesis Inhibitors / therapeutic use
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Animals
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Antineoplastic Agents, Phytogenic / pharmacology*
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Antineoplastic Agents, Phytogenic / therapeutic use
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Carcinoma, Squamous Cell / blood supply
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Carcinoma, Squamous Cell / drug therapy
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Carcinoma, Squamous Cell / pathology*
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Cell Line, Tumor / drug effects
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Cell Line, Tumor / metabolism
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Cell Line, Tumor / transplantation
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Cells, Cultured / drug effects
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Drug Resistance, Neoplasm / drug effects
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Drugs, Chinese Herbal / pharmacology*
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Drugs, Chinese Herbal / therapeutic use
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Endothelium, Vascular / cytology
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Epidermal Growth Factor / pharmacology
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ErbB Receptors / biosynthesis*
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ErbB Receptors / genetics
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ErbB Receptors / physiology
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Ganoderma / chemistry*
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Gene Expression Regulation, Neoplastic / drug effects
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Humans
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Methanol
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism
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Neovascularization, Pathologic / drug therapy*
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Neovascularization, Pathologic / physiopathology
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Neovascularization, Physiologic / drug effects
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Phytotherapy*
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Reishi
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Skin Neoplasms / blood supply
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Skin Neoplasms / drug therapy
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Skin Neoplasms / pathology*
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Vascular Endothelial Growth Factor A / biosynthesis*
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Vascular Endothelial Growth Factor A / genetics
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Vascular Endothelial Growth Factor A / metabolism
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Xenograft Model Antitumor Assays
Substances
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Angiogenesis Inhibitors
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Antineoplastic Agents, Phytogenic
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Drugs, Chinese Herbal
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Neoplasm Proteins
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Plant Extracts
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VEGFA protein, human
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Vascular Endothelial Growth Factor A
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Epidermal Growth Factor
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EGFR protein, human
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ErbB Receptors
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Methanol