Abstract
Homeodomain transcription factor Nanog plays a critical role in maintaining murine embryonic stem (ES) cells pluripotency. However, its expression regulation largely remains unknown. In this study we show that Nogo receptor (NgR) participates in the regulation of Nanog expression via Stat3 pathway. Activation of NgR results in the phosphorylation of Stat3 and increases expression levels of Nanog mRNA and protein, which inhibits differentiation of embryoid bodies. This up-regulation of Nanog can be abolished by NgR inhibitor PI-PLC and NEP1-40, or phospho-Stat3 inhibitor AG490 and rapamycin. Immunofluorescence assay demonstrates that NgR and its ligand Nogo-A/B exist on mouse blastocysts and cultured ES cells, suggesting NgR might play a role in early embryo development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / drug effects
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Cell Differentiation / drug effects
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Cell Differentiation / genetics
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Cell Proliferation / drug effects
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Cells, Cultured
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Embryonic Stem Cells / drug effects
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Embryonic Stem Cells / metabolism*
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Female
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GPI-Linked Proteins
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Gene Expression Regulation, Developmental / drug effects
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Homeodomain Proteins / genetics*
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Homeodomain Proteins / metabolism
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Mice
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Myelin Proteins / genetics
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Myelin Proteins / metabolism
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Myelin Proteins / pharmacology
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Myelin Proteins / physiology*
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Nanog Homeobox Protein
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Nogo Proteins
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Nogo Receptor 1
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Pregnancy
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism
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Receptors, Cell Surface / physiology
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STAT3 Transcription Factor / metabolism
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STAT3 Transcription Factor / physiology*
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Signal Transduction / genetics
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Signal Transduction / physiology
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Time Factors
Substances
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GPI-Linked Proteins
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Homeodomain Proteins
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Myelin Proteins
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Nanog Homeobox Protein
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Nanog protein, mouse
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Nogo Proteins
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Nogo Receptor 1
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Receptors, Cell Surface
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Rtn4 protein, mouse
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Rtn4r protein, mouse
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STAT3 Transcription Factor
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Stat3 protein, mouse