Abstract
Although green tea polyphenol catechin is considered as a potential anti-inflammatory agent, its effect on bacterial component-induced inflammation has been poorly investigated. We examined the capacity of epigallocatechin gallate (EGCG) to regulate leukocyte responses to bacterial chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (fMLF), which is recognized by a human G protein-coupled receptor FPR on phagocytic leukocytes. Pretreatment of human monocytic cells or FPR-transfected rat basophilic leukemia cells (ETFR cells) with EGCG significantly inhibited fMLF-induced chemotaxis. Intraperitoneal administration of EGCG in mice suppressed fMLF-induced leukocyte infiltration into the air pouch created in the skin. Mechanistic studies revealed that EGCG dose-dependently suppressed fMLF-induced calcium flux in monocytic cells and ETFR cells. fMLF-induced ETFR cell migration was significantly inhibited by a specific MEK1/2 inhibitor, PD98059, which was associated with reduction in fMLF-induced ERK1/2 phosphorylation. These results suggest that EGCG inhibits FPR-mediated leukocyte activation thus is a promising anti-inflammatory compound.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / pharmacology*
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Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors
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Camellia sinensis / immunology
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Catechin / analogs & derivatives*
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Catechin / pharmacology
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Cell Line, Tumor
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Cell Movement / drug effects
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Cell Movement / immunology
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Flavonoids / pharmacology
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Humans
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Inflammation
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Injections, Intraperitoneal
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Leukemia, Basophilic, Acute / blood
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Leukemia, Basophilic, Acute / drug therapy
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Leukemia, Basophilic, Acute / immunology
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Mice
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Monocytes / drug effects
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Monocytes / immunology
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Monocytes / metabolism*
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N-Formylmethionine Leucyl-Phenylalanine / immunology
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N-Formylmethionine Leucyl-Phenylalanine / metabolism*
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Rats
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Receptors, Formyl Peptide / genetics
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Receptors, Formyl Peptide / immunology
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Receptors, Formyl Peptide / metabolism
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Signal Transduction / drug effects
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Signal Transduction / immunology
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Transfection
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Transgenes
Substances
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Antioxidants
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Flavonoids
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Receptors, Formyl Peptide
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N-Formylmethionine Leucyl-Phenylalanine
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Catechin
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epigallocatechin gallate
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Calcium-Calmodulin-Dependent Protein Kinases
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2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one