Abstract
The detection of an increasing number of cytokines and the demonstration of autocrine and paracrine mechanisms perpetuating tumor growth prompted the investigation of the expression of the cytokines IL-2, IL-3, IL-4, IL-5, IL-6, IFN gamma, Tac, and GMCSF in primary lymph-node biopsies of patients with peripheral T-cell lymphoma (n = 11), Hodgkin's disease (n = 13), and large-cell anaplastic lymphoma (n = 6) by means of Northern blot analysis and in situ hybridization (ISH); 15 of 28 cases had IL-6 message, predominantly in cases of Hodgkin's disease (HD) and large-cell anaplastic lymphomas (LCAL). Interferon gamma was found in about 50% of the cases among all entities. Other cytokine expression was rare except two cases of HD with high amounts of IL-4 mRNA. These results indicate that large amounts of growth factor transcripts are present in a variety of malignant lymphomas. The meaning of this expression is still unclear. It may be a loss of physiologic regulation within the cytokine network which may thus influence neoplastic cell growth as some cases have a quantity of cytokine expression which is similar or even above that of stimulated T cells. ISH demonstrates in individual cases that the expression is at least in part due to malignant cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal
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Biopsy
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Blotting, Northern
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Cell Transformation, Neoplastic / genetics
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Cell Transformation, Neoplastic / metabolism*
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Cell Transformation, Neoplastic / pathology
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Cytokines / genetics
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Cytokines / metabolism*
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Gene Expression
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Granulocyte-Macrophage Colony-Stimulating Factor / genetics
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Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
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Hodgkin Disease / genetics
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Hodgkin Disease / metabolism*
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Hodgkin Disease / pathology
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Humans
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Interferon-gamma / genetics
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Interferon-gamma / metabolism
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Interleukin-2 / genetics
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Interleukin-2 / metabolism
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Interleukin-3 / genetics
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Interleukin-3 / metabolism
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Interleukin-4 / genetics
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Interleukin-4 / metabolism
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Interleukin-5 / genetics
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Interleukin-5 / metabolism
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Interleukin-6 / genetics
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Interleukin-6 / metabolism
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Lymph Nodes / metabolism
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Lymph Nodes / pathology
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Lymphoma, T-Cell / genetics
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Lymphoma, T-Cell / metabolism*
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Nucleic Acid Hybridization
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, Interleukin-2 / genetics
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Receptors, Interleukin-2 / metabolism
Substances
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Antibodies, Monoclonal
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Cytokines
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Interleukin-2
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Interleukin-3
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Interleukin-5
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Interleukin-6
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RNA, Messenger
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Receptors, Interleukin-2
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Interleukin-4
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Interferon-gamma
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Granulocyte-Macrophage Colony-Stimulating Factor