Abstract
The sequence was determined of 6493 nucleotides encompassing the bet genes of Escherichia coli which encode the osmoregulatory choline-glycine betaine pathway. Four open reading frames were identified: betA encoding choline dehydrogenase, a flavoprotein of 61.9kDa; betB encoding betaine aldehyde dehydrogenase (52.8kDa); betT encoding a proton-motive-force-driven, high-affinity transport system for choline (75.8kDa); and betl, capable of encoding a protein of 21.8kDa, implicated as a repressor involved in choline regulation of the bet genes. Identification of the genes was supported by subcloning, physical mapping of lambda placMu53 insertions, amino acid sequence similarity, or N-terminal amino acid sequencing. The bet genes are tightly spaced, with betT located upstream of, and transcribed divergently to, the tandemly linked betIBA genes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alcohol Oxidoreductases / genetics*
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Alcohol Oxidoreductases / metabolism
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Aldehyde Oxidoreductases / genetics*
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Aldehyde Oxidoreductases / metabolism
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Amino Acid Sequence
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Betaine-Aldehyde Dehydrogenase
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Biological Transport
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Choline / metabolism*
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Choline Dehydrogenase
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Gene Expression Regulation, Bacterial
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Genes, Bacterial*
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Glycine / metabolism*
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Molecular Sequence Data
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Open Reading Frames
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Osmotic Pressure
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Sequence Homology, Nucleic Acid
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Transcription, Genetic
Substances
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Bacterial Proteins
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Recombinant Fusion Proteins
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Repressor Proteins
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Alcohol Oxidoreductases
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Choline Dehydrogenase
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Aldehyde Oxidoreductases
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Betaine-Aldehyde Dehydrogenase
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Choline
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Glycine
Associated data
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GENBANK/D12728
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GENBANK/D12729
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GENBANK/D12730
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GENBANK/D12731
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GENBANK/D12732
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GENBANK/D12733
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GENBANK/S67313
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GENBANK/S67318
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GENBANK/X17677
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GENBANK/X17678
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GENBANK/X52905