DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli

Mol Microbiol. 1991 May;5(5):1049-64. doi: 10.1111/j.1365-2958.1991.tb01877.x.

Abstract

The sequence was determined of 6493 nucleotides encompassing the bet genes of Escherichia coli which encode the osmoregulatory choline-glycine betaine pathway. Four open reading frames were identified: betA encoding choline dehydrogenase, a flavoprotein of 61.9kDa; betB encoding betaine aldehyde dehydrogenase (52.8kDa); betT encoding a proton-motive-force-driven, high-affinity transport system for choline (75.8kDa); and betl, capable of encoding a protein of 21.8kDa, implicated as a repressor involved in choline regulation of the bet genes. Identification of the genes was supported by subcloning, physical mapping of lambda placMu53 insertions, amino acid sequence similarity, or N-terminal amino acid sequencing. The bet genes are tightly spaced, with betT located upstream of, and transcribed divergently to, the tandemly linked betIBA genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcohol Oxidoreductases / genetics*
  • Alcohol Oxidoreductases / metabolism
  • Aldehyde Oxidoreductases / genetics*
  • Aldehyde Oxidoreductases / metabolism
  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Betaine-Aldehyde Dehydrogenase
  • Biological Transport
  • Choline / metabolism*
  • Choline Dehydrogenase
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial*
  • Glycine / metabolism*
  • Molecular Sequence Data
  • Open Reading Frames
  • Osmotic Pressure
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / genetics*
  • Repressor Proteins / metabolism
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Alcohol Oxidoreductases
  • Choline Dehydrogenase
  • Aldehyde Oxidoreductases
  • Betaine-Aldehyde Dehydrogenase
  • Choline
  • Glycine

Associated data

  • GENBANK/D12728
  • GENBANK/D12729
  • GENBANK/D12730
  • GENBANK/D12731
  • GENBANK/D12732
  • GENBANK/D12733
  • GENBANK/S67313
  • GENBANK/S67318
  • GENBANK/X17677
  • GENBANK/X17678
  • GENBANK/X52905