Abstract
The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex. We have isolated human complementary DNA clones for both the subunits of TFIIE. Using purified recombinant proteins we find that both subunits are essential to form a stable preinitiation complex and to reconstitute basal-level and Sp1-activated transcription in vitro. Analysis of their predicted amino-acid sequences reveals several intriguing structural motifs that could provide insight into the role of TFIIE in transcription initiation.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphate / metabolism
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA / genetics
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DNA-Binding Proteins / physiology
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Escherichia coli
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Humans
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Macromolecular Substances
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Molecular Sequence Data
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Protein Binding
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RNA Polymerase II / physiology
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Recombinant Proteins
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Sequence Homology, Nucleic Acid
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Transcription Factors / physiology*
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Transcription Factors, TFII*
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Transcription, Genetic*
Substances
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DNA-Binding Proteins
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Macromolecular Substances
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Recombinant Proteins
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Transcription Factors
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Transcription Factors, TFII
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transcription factor TFIIE
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Adenosine Triphosphate
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DNA
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RNA Polymerase II
Associated data
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GENBANK/S67859
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GENBANK/S67861
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GENBANK/S68227
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GENBANK/X57452
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GENBANK/X57453
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GENBANK/X57454
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GENBANK/X57455
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GENBANK/X62895
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GENBANK/X62896
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GENBANK/X62897