Abstract
The aim of the study was to compare a novel, rolling circle amplification (RCA) assay for detection of common isoniazid (INH) resistance mutations in Mycobacterium tuberculosis with a multiplex allele-specific PCR (MAS-PCR) and sequencing of katG and the fabG1-inhA promoter region. One or more mutations were identified by RCA, MAS-PCR, and sequencing in 21 (68%), 22 (71%), and 23 (74%), respectively, of 31 epidemiologically unrelated INH-resistant isolates, and in none of 8 INH-susceptible isolates. The RCA assay is a rapid, inexpensive, and practical screening method for INH resistance in M. tuberculosis in countries with high prevalence of INH resistance.
MeSH terms
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Alleles
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Bacterial Proteins / genetics*
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Catalase / genetics*
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Drug Resistance, Bacterial*
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Humans
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Microbial Sensitivity Tests / economics
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Microbial Sensitivity Tests / methods*
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Mutation, Missense*
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Mycobacterium tuberculosis / drug effects*
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / isolation & purification
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Nucleic Acid Amplification Techniques*
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Oxidoreductases / genetics*
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Polymerase Chain Reaction / economics
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Polymerase Chain Reaction / methods*
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Promoter Regions, Genetic
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Sequence Analysis, DNA
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Time Factors
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Tuberculosis / microbiology
Substances
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Bacterial Proteins
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Oxidoreductases
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Catalase
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katG protein, Mycobacterium tuberculosis
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InhA protein, Mycobacterium