Abstract
Interaction between cell surface integrin receptors and extracellular matrix (ECM) components plays an important role in cell survival, proliferation, and migration, including tumor development and invasion of tumor cells. Matrix metalloproteinases (MMPs) are a family of metalloproteinases capable of digesting ECM components and are important molecules for cell migration. Binding of ECM to integrins initiates cascades of cell signaling events modulating expression and activity of different MMPs. The aim of this study is to investigate fibronectin-integrin-mediated signaling and modulation of MMPs. Our findings indicated that culture of human cervical cancer cell (SiHa) on fibronectin-coated surface perhaps sends signals via fibronectin-integrin-mediated signaling pathways recruiting focal adhesion kinase (FAK) extracellular signal regulated kinase (ERK), phosphatidyl inositol 3 kinase (PI-3K), integrin-linked kinase (ILK), nuclear factor-kappa B (NF-kappaB), and modulates expression and activation of mainly pro-MMP-9, and moderately pro-MMP-2 in serum-free culture medium.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line, Tumor
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Cell Movement
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Female
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Fibronectins / metabolism*
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Focal Adhesion Protein-Tyrosine Kinases / genetics
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Focal Adhesion Protein-Tyrosine Kinases / metabolism
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Gene Expression Regulation, Neoplastic*
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Humans
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Integrin alpha5beta1 / metabolism*
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Matrix Metalloproteinase 2 / genetics
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Matrix Metalloproteinase 2 / metabolism
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Matrix Metalloproteinase 9 / genetics
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Matrix Metalloproteinase 9 / metabolism*
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NF-kappa B / metabolism
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Phosphatidylinositol 3-Kinases / metabolism
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Phosphorylation
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Protein Serine-Threonine Kinases / metabolism
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Protein Transport
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RNA, Messenger / metabolism
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Signal Transduction*
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Tissue Inhibitor of Metalloproteinases / genetics
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Tissue Inhibitor of Metalloproteinases / metabolism
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Uterine Cervical Neoplasms / genetics
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Uterine Cervical Neoplasms / metabolism*
Substances
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Fibronectins
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Integrin alpha5beta1
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NF-kappa B
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RNA, Messenger
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Tissue Inhibitor of Metalloproteinases
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integrin-linked kinase
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Focal Adhesion Protein-Tyrosine Kinases
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Protein Serine-Threonine Kinases
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Extracellular Signal-Regulated MAP Kinases
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9