Conversion of peripheral CD4(+)CD25(-) T cells to CD4(+)CD25(+) regulatory T cells by IFN-gamma in patients with Guillain-Barré syndrome

J Neuroimmunol. 2009 Dec 10;217(1-2):80-4. doi: 10.1016/j.jneuroim.2009.10.001. Epub 2009 Oct 24.

Abstract

CD4(+)CD25(-) T cells from 25 Guillain-Barré syndrome (GBS) patients were stimulated by IFN-gamma to generate the induced CD4(+)CD25(+) regulatory T cells (iTregs). Then, their phenotypical and functional characterizations were analyzed. Results show that iTregs can be generated from CD4(+)CD25(-) T cells in GBS patients by IFN-gamma in the presence of activating anti-CD3 and anti-CD28 antibodies in vitro. When CD4(+)CD25(-) T cells are stimulated by 40ng/ml IFN-gamma for 3days, the ratio of conversion is the highest under this condition and iTregs are similar to naturally occurring CD4(+)CD25(+) regulatory T cells of healthy donors phenotypically and functionally.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Antiviral Agents / pharmacology*
  • CD4 Antigens / metabolism
  • Cell Proliferation / drug effects
  • Female
  • Flow Cytometry / methods
  • Forkhead Transcription Factors / genetics
  • Forkhead Transcription Factors / metabolism
  • Gene Expression Regulation / drug effects
  • Guillain-Barre Syndrome / immunology*
  • Guillain-Barre Syndrome / pathology*
  • Humans
  • Interferon-gamma / pharmacology*
  • Interleukin-2 Receptor alpha Subunit / metabolism
  • Lymphocyte Activation / drug effects*
  • Male
  • Middle Aged
  • T-Lymphocytes, Regulatory / immunology*
  • T-Lymphocytes, Regulatory / metabolism
  • Young Adult

Substances

  • Antiviral Agents
  • CD4 Antigens
  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • Interleukin-2 Receptor alpha Subunit
  • Interferon-gamma