Abstract
CD4(+)CD25(-) T cells from 25 Guillain-Barré syndrome (GBS) patients were stimulated by IFN-gamma to generate the induced CD4(+)CD25(+) regulatory T cells (iTregs). Then, their phenotypical and functional characterizations were analyzed. Results show that iTregs can be generated from CD4(+)CD25(-) T cells in GBS patients by IFN-gamma in the presence of activating anti-CD3 and anti-CD28 antibodies in vitro. When CD4(+)CD25(-) T cells are stimulated by 40ng/ml IFN-gamma for 3days, the ratio of conversion is the highest under this condition and iTregs are similar to naturally occurring CD4(+)CD25(+) regulatory T cells of healthy donors phenotypically and functionally.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Antiviral Agents / pharmacology*
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CD4 Antigens / metabolism
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Cell Proliferation / drug effects
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Female
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Flow Cytometry / methods
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Forkhead Transcription Factors / genetics
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Forkhead Transcription Factors / metabolism
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Gene Expression Regulation / drug effects
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Guillain-Barre Syndrome / immunology*
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Guillain-Barre Syndrome / pathology*
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Humans
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Interferon-gamma / pharmacology*
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Interleukin-2 Receptor alpha Subunit / metabolism
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Lymphocyte Activation / drug effects*
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Male
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Middle Aged
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T-Lymphocytes, Regulatory / immunology*
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T-Lymphocytes, Regulatory / metabolism
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Young Adult
Substances
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Antiviral Agents
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CD4 Antigens
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FOXP3 protein, human
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Forkhead Transcription Factors
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Interleukin-2 Receptor alpha Subunit
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Interferon-gamma