The growth factor independence-1 (Gfi1) is overexpressed in chronic myelogenous leukemia

Acta Haematol. 2010;123(1):1-5. doi: 10.1159/000253856. Epub 2009 Nov 2.

Abstract

The activation of ABL tyrosine kinase in BCR/ABL-positive chronic myelogenous leukemia (CML) leads to a diversity of biological changes related to the pathogenesis of the disease. In CML patients, we determined the expression of growth factor independence-1 (Gfi1), a transcription repressor with weak oncogenic activity. Our data demonstrated that the Gfi1 mRNA level in both the mononuclear cells and purified CD34(+) cells from CML were significantly higher as measured by quantitative real-time PCR. Using flow cytometry and Western blot, we also showed that the Gfi1 protein content was increased in CML CD34(+) cells. The expression of Gfi1 was correlated with BCR/ABL significantly. Gfi1 may be implicated in the pathogenesis of CML and can serve as a potential target for the management of the disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD34 / metabolism
  • Base Sequence
  • Benzamides
  • Blast Crisis / genetics
  • Blast Crisis / metabolism
  • DNA Primers / genetics
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Gene Expression
  • Genes, abl
  • Hematopoietic Stem Cells / immunology
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Imatinib Mesylate
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / immunology
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / therapy
  • Leukemia, Myeloid, Accelerated Phase / genetics
  • Leukemia, Myeloid, Accelerated Phase / metabolism
  • Leukemia, Myeloid, Chronic-Phase / genetics
  • Leukemia, Myeloid, Chronic-Phase / metabolism
  • Neoplastic Stem Cells / immunology
  • Neoplastic Stem Cells / metabolism
  • Piperazines / therapeutic use
  • Pyrimidines / therapeutic use
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism
  • Stem Cell Transplantation
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*

Substances

  • Antigens, CD34
  • Benzamides
  • DNA Primers
  • DNA-Binding Proteins
  • GFI1 protein, human
  • Piperazines
  • Pyrimidines
  • RNA, Messenger
  • RNA, Neoplasm
  • Transcription Factors
  • Imatinib Mesylate