Abstract
Many RNA and DNA viruses activate serine-threonine kinase AKT to increase virus replication. In contrast, measles virus (MV) infection leads to downregulation of AKT. This is thought to be beneficial for the virus because it correlates with immune suppression. To determine whether this is a sacrifice for the virus, we used a recombinant virus and transfected cells expressing constitutively active AKT and evaluated its effect on virus replication. In vitro, overexpression of AKT did not influence virus replication but did affect (cell-type dependent) virus release. In vivo, the recombinant virus did not abrogate inhibition of proliferation of spleen cells from MV-infected cotton rats.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Chlorocebus aethiops
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Host-Pathogen Interactions / genetics
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Host-Pathogen Interactions / physiology
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Humans
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Jurkat Cells
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Measles / pathology
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Measles / virology
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Measles virus / genetics
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Measles virus / pathogenicity
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Measles virus / physiology*
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Proto-Oncogene Proteins c-akt / genetics
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Proto-Oncogene Proteins c-akt / physiology*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Recombination, Genetic
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Sigmodontinae
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Spleen / pathology
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Spleen / virology
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Transfection
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Vero Cells
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Virus Replication / genetics
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Virus Replication / physiology*
Substances
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Recombinant Proteins
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Proto-Oncogene Proteins c-akt