Abstract
In an effort to target the in vivo context of tumor-specific moieties, we screened a large library of nuclease-resistant RNA oligonucleotides in tumor-bearing mice to identify candidate molecules with the ability to localize to hepatic colon cancer metastases. One of the selected molecules is an RNA aptamer that binds to p68, an RNA helicase that has been shown to be upregulated in colorectal cancer.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Aptamers, Nucleotide / chemistry
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COS Cells
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Cell Line, Tumor
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Chlorocebus aethiops
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Colonic Neoplasms / pathology*
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Colonic Neoplasms / therapy*
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DEAD-box RNA Helicases / metabolism*
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Gene Expression Regulation, Neoplastic*
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Genetic Techniques
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Humans
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Kinetics
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Liver Neoplasms / secondary*
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Liver Neoplasms / therapy*
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Mice
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Neoplasm Metastasis
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Neoplasms / genetics*
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Neoplasms / therapy*
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Oligonucleotides / chemistry
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RNA / metabolism*
Substances
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Aptamers, Nucleotide
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Oligonucleotides
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RNA
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DEAD-box RNA Helicases