Abstract
Human c-Rel (REL) is a member of the NF-kappaB family of transcription factors. REL's normal physiological role is in the regulation of B-cell proliferation and survival. The REL gene is amplified in many human B-cell lymphomas and overexpression of REL can transform chicken lymphoid cells. In this report, histone acetyltransferase p300 enhanced REL-induced transactivation and interacted with REL both in vitro and in REL-transformed chicken spleen cells and the B-lymphoma cell line RC-K8, in which REL is constitutively active and required for proliferation. However, due to a deletion in the EP300 locus, only a C-terminally truncated form of p300 is expressed in RC-K8 cells. These results suggest a role for p300 in REL-mediated oncogenic activity in B lymphoma.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cell Line, Tumor
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Cell Transformation, Neoplastic
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Cells, Cultured
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Chickens
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Genes, Reporter
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Glutathione Transferase / genetics
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Glutathione Transferase / metabolism
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Histone Acetyltransferases / metabolism*
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Humans
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Kidney / cytology
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Kidney / embryology
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Luciferases / genetics
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Lymphoma, B-Cell / genetics
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Lymphoma, B-Cell / metabolism
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Lymphoma, B-Cell / pathology
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Lymphoma, Large B-Cell, Diffuse / genetics*
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Lymphoma, Large B-Cell, Diffuse / metabolism
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Lymphoma, Large B-Cell, Diffuse / pathology
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NF-kappa B / genetics
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NF-kappa B / metabolism
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Polymerase Chain Reaction
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Proto-Oncogene Proteins c-rel / deficiency
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Proto-Oncogene Proteins c-rel / genetics
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Proto-Oncogene Proteins c-rel / metabolism*
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Recombinant Fusion Proteins / metabolism
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Transfection
Substances
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NF-kappa B
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Proto-Oncogene Proteins c-rel
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Recombinant Fusion Proteins
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Luciferases
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Histone Acetyltransferases
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Glutathione Transferase