Allergy-related cytokines (IL-4 and TNF-α) are induced by Di(2-ethylhexyl) phthalate and attenuated by plant-originated glycoprotein (75 kDa) in HMC-1 cells

Jin Lee; Phil-Sun Oh; Kye-Taek Lim

doi:10.1002/tox.20563

Allergy-related cytokines (IL-4 and TNF-α) are induced by Di(2-ethylhexyl) phthalate and attenuated by plant-originated glycoprotein (75 kDa) in HMC-1 cells

Environ Toxicol. 2011 Aug;26(4):364-72. doi: 10.1002/tox.20563. Epub 2010 Jan 15.

Authors

Jin Lee¹, Phil-Sun Oh, Kye-Taek Lim

Affiliation

¹ Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, 300 Yongbong-Dong, Gwang-ju 500-757, South Korea.

PMID: 20082445
DOI: 10.1002/tox.20563

Abstract

Phthalate esters as plasticizers have been widespread in the environment and may be associated with development of allergic diseases such as asthma and atopic dermatitis. In this study, we demonstrated that the CTB glycoprotein attenuates allergic reactions caused by di(2-ethylhexyl) phthalate (DEHP) in human mast cells (HMC-1). This experiment evaluated degranulation of histamine and β-hexosaminidase as well as activities of protein kinase C (PKC), stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), activator protein (AP)-1 and interleukin (IL)-4 and tumor necrosis factor (TNF)-α using immunoblotting and reverse transcription-polymerase chain reaction (RT-PCR). Our results revealed that the CTB glycoprotein in the presence of DEHP inhibits degranulation of mast cell, translocation of PKC from cytosol to membrane, and phosphorylation of SAPK/JNK in HMC -1 cells. We also found that the CTB glycoprotein (100 μg mL(-1) ) has suppressive effects on transcriptional activation of AP-1, and on the expression of IL-4 and TNF-α in DEHP-treated HMC-1 cells. We suggest that the CTB glycoprotein inhibits degranulation of mast cells and expressions of cytokines in HMC-1 cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Allergic Agents / pharmacology*
Cell Line
Diethylhexyl Phthalate / toxicity*
Estrogens / toxicity*
Glycoproteins / pharmacology*
Histamine / metabolism
Humans
Hypersensitivity / drug therapy
Hypersensitivity / metabolism*
Interleukin-4 / antagonists & inhibitors
Interleukin-4 / genetics
Interleukin-4 / metabolism
JNK Mitogen-Activated Protein Kinases / antagonists & inhibitors
JNK Mitogen-Activated Protein Kinases / metabolism
Mast Cells
Moraceae / metabolism
Phosphorylation / drug effects
Plant Extracts / pharmacology
Plant Proteins / pharmacology*
Protein Kinase C / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factor AP-1 / metabolism
Tumor Necrosis Factor-alpha / antagonists & inhibitors
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / metabolism
beta-N-Acetylhexosaminidases / antagonists & inhibitors
beta-N-Acetylhexosaminidases / metabolism

Substances

Anti-Allergic Agents
Estrogens
Glycoproteins
Plant Extracts
Plant Proteins
Transcription Factor AP-1
Tumor Necrosis Factor-alpha
Interleukin-4
Histamine
Diethylhexyl Phthalate
Protein Kinase C
JNK Mitogen-Activated Protein Kinases
beta-N-Acetylhexosaminidases