Expression of the c-fes proto-oncogene in granulocyte-macrophage colony-stimulating factor-dependent acute myelogenous leukemia cells grown autonomously

Int J Cell Cloning. 1991 Jan;9(1):89-94. doi: 10.1002/stem.5530090111.

Abstract

In the present article, we show that 6 of 69 acute myelogenous leukemia (AML) samples exhibited autonomous in vitro growth that was dependent on endogenous granulocyte-macrophage colony-stimulating factor (GM-CSF). Cytoplasmic RNA harvested from all 6 leukemia specimens contained GM-CSF transcripts easily detectable by mRNA hybridization. All 6 GM-CSF-expressing leukemia samples simultaneously displayed high levels of transcripts for the c-fes proto-oncogene previously shown to be expressed in GM-CSF sensitive myeloid cells, whereas only 2 of the 48 AML samples not expressing GM-CSF accumulated c-fes mRNA. Seven of additional 14 GM-CSF-expressing specimens showed specific signals upon RNA hybridization with the c-fes probe, but failed to grow autonomously. These results suggest that c-fes and GM-CSF genes may be coordinately regulated in AML blasts and that GM-CSF-mediated growth autonomy may be linked to c-fes expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division
  • Gene Expression
  • Granulocyte-Macrophage Colony-Stimulating Factor / genetics
  • Granulocyte-Macrophage Colony-Stimulating Factor / physiology*
  • Humans
  • Leukemia, Myeloid, Acute / genetics*
  • Leukemia, Myeloid, Acute / metabolism
  • Neoplasm Proteins / genetics
  • Protein-Tyrosine Kinases*
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-fes
  • Proto-Oncogenes*
  • RNA, Neoplasm / analysis
  • Tumor Cells, Cultured

Substances

  • MAS1 protein, human
  • Neoplasm Proteins
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins
  • RNA, Neoplasm
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Protein-Tyrosine Kinases
  • FES protein, human
  • Proto-Oncogene Proteins c-fes