Abstract
Ascorbic acid is a sugar acid and an essential vital food nutrient found mainly in fruits and vegetables. The purpose of this study was to investigate the effects of ascorbic acid against arsenic induced oxidative stress in blood of rat. In rat, treatment with ascorbic acid prevented the increased serum enzymatic activity of AST, ALT, ALP, ACP and LDH. In addition, treatment with ascorbic acid prevented elevated production of LPO, PC and NO and restored the depletion of reduced SOD and CAT activities. Interestingly, ascorbic acid markedly upregulated lymphocytes relative mRNA expression of lymphocytes SOD2 gene corresponding to GAPDH, house keeping candidate gene in arsenic-treated rat, which might provide anti-oxidative activity in the blood.
2010 Elsevier Ltd. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antioxidants / pharmacology*
-
Arsenic Poisoning / metabolism
-
Arsenic Poisoning / prevention & control*
-
Ascorbic Acid / pharmacology*
-
Biomarkers / blood
-
Catalase / metabolism
-
Chronic Disease
-
Disease Models, Animal
-
Enzymes / blood
-
Gene Expression Regulation, Enzymologic / drug effects
-
Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
-
Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
-
Lipid Peroxidation / drug effects
-
Lymphocytes / chemistry
-
Lymphocytes / drug effects
-
Male
-
Nitric Oxide / metabolism
-
Oxidative Stress / drug effects*
-
RNA, Messenger / metabolism
-
Rats
-
Rats, Wistar
-
Superoxide Dismutase / genetics
-
Superoxide Dismutase / metabolism
-
Up-Regulation / drug effects
Substances
-
Antioxidants
-
Biomarkers
-
Enzymes
-
RNA, Messenger
-
Nitric Oxide
-
Catalase
-
Superoxide Dismutase
-
superoxide dismutase 2
-
Glyceraldehyde-3-Phosphate Dehydrogenases
-
Ascorbic Acid