Oxidized low-density lipoprotein-receptor 1 (OLR-1) might be involved in the risk of atherosclerosis and its complications. Several studies have been carried out to explore the role of OLR-1 gene polymorphisms in the risk of coronary artery disease. Our study investigated whether the G501C and the 3'UTR C188T polymorphisms of the OLR-1 gene were genetic risk factors of acute coronary syndrome (ACS) in the Han Chinese population. Significant differences were found in genotype frequencies of the OLR-1 G501C polymorphism between 198 ACS patients and 204 control individuals. The CC genotype frequency in the ACS group was significantly higher than that in the control group (5.6% and 1.5% in ACS patients and control subjects, respectively), and the p-value and odds ratio were 0.028 and 3.911, respectively. Using the CC genotype as a reference, we have shown a significant association between carriers of the G allele (GG + GC genotypes) and a decreased risk of ACS (p = 0.026, odds ratio = 0.254, 95% confidence interval = 0.070-0.924). The OLR-1 G501C polymorphism might be associated with ACS in the Han Chinese population.