Diminished lipocalin-type prostaglandin D(2) synthase expression in human lung tumors

Louis Ragolia; Thomas Palaia; Christopher E Hall; Jonathan Klein; Arzu Büyük

doi:10.1016/j.lungcan.2010.01.011

Diminished lipocalin-type prostaglandin D(2) synthase expression in human lung tumors

Lung Cancer. 2010 Oct;70(1):103-9. doi: 10.1016/j.lungcan.2010.01.011. Epub 2010 Feb 7.

Authors

Louis Ragolia¹, Thomas Palaia, Christopher E Hall, Jonathan Klein, Arzu Büyük

Affiliation

¹ Vascular Biology Institute, Winthrop-University Hospital, Mineola, NY 11501, USA. LRagolia@Winthrop.org

PMID: 20144489
DOI: 10.1016/j.lungcan.2010.01.011

Abstract

Previously, we demonstrated that lipocalin-type prostaglandin D(2) synthase (L-PGDS) induces apoptosis and prevents cell cycle progression in several cell types. In this study we determined the expression of L-PGDS in a variety of human lung tumor types. While L-PGDS expression was evident in the surrounding margins, we observed significantly decreased protein and gene expression in the tumor tissue. Using RT-PCR we demonstrated that L-PGDS gene expression decreased proportionately with tumor progression. In addition, we demonstrated that exogenously added L-PGDS could suppress the hyperproliferation and PDGF-stimulated migration of A549 cells, a cultured carcinomic human alveolar basal epithelial cell line. We conclude that L-PGDS may play a key role in modulating lung cancer growth and may offer a novel diagnostic and therapeutic approach for treatment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma, Bronchiolo-Alveolar / enzymology
Adenocarcinoma, Bronchiolo-Alveolar / genetics
Adenocarcinoma, Bronchiolo-Alveolar / pathology
Apoptosis / drug effects
Apoptosis / physiology
Carcinoma, Non-Small-Cell Lung / enzymology
Carcinoma, Non-Small-Cell Lung / genetics
Carcinoma, Non-Small-Cell Lung / pathology
Cell Growth Processes / drug effects
Cell Growth Processes / physiology
Cell Line, Tumor
Cell Movement / drug effects
Cell Movement / physiology
Disease Progression
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Humans
Intramolecular Oxidoreductases / biosynthesis*
Intramolecular Oxidoreductases / deficiency
Intramolecular Oxidoreductases / genetics
Intramolecular Oxidoreductases / pharmacology
Lipocalins / biosynthesis*
Lipocalins / genetics
Lipocalins / pharmacology
Lung Neoplasms / enzymology*
Lung Neoplasms / genetics
Lung Neoplasms / pathology
Platelet-Derived Growth Factor / pharmacology
Reverse Transcriptase Polymerase Chain Reaction

Substances

Lipocalins
Platelet-Derived Growth Factor
Intramolecular Oxidoreductases
prostaglandin R2 D-isomerase