Abstract
A 4.8 kb DNA-fragment was cloned and sequenced encompassing the structural gene of PFL-deactivase (2.7 kb) and 2 kb of the 5' flanking region that contains the elements for anaerobic induction. A mutant lacking deactivase was shown to require exogenous electron acceptors for anaerobic growth with glucose. This revealed the identity of PFL-deactivase with the alcohol and acetaldehyde dehydrogenases of E. coli. The multienzyme represents a homopolymeric protein (approximately 40 x 96 kDa) requiring Fe2+ for all functions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Dehydrogenase / genetics*
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Alcohol Dehydrogenase / isolation & purification
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Alcohol Dehydrogenase / metabolism
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Aldehyde Oxidoreductases / genetics*
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Aldehyde Oxidoreductases / isolation & purification
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Aldehyde Oxidoreductases / metabolism
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Anaerobiosis
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Base Sequence
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Escherichia coli / growth & development
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Escherichia coli Proteins
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Genes, Bacterial*
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Kinetics
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Molecular Sequence Data
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Multienzyme Complexes / genetics*
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Multienzyme Complexes / isolation & purification
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Multienzyme Complexes / metabolism
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Plasmids
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Restriction Mapping
Substances
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Escherichia coli Proteins
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Multienzyme Complexes
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Alcohol Dehydrogenase
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adhE protein, E coli
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Aldehyde Oxidoreductases
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acetaldehyde dehydrogenase (acylating)
Associated data
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GENBANK/X58797
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GENBANK/X58798
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GENBANK/X58799
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GENBANK/X58800
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GENBANK/X58801
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GENBANK/X58802
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GENBANK/X58803
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GENBANK/X59501
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GENBANK/X59502
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GENBANK/X62841