Pyruvate-formate-lyase-deactivase and acetyl-CoA reductase activities of Escherichia coli reside on a polymeric protein particle encoded by adhE

FEBS Lett. 1991 Apr 9;281(1-2):59-63. doi: 10.1016/0014-5793(91)80358-a.

Abstract

A 4.8 kb DNA-fragment was cloned and sequenced encompassing the structural gene of PFL-deactivase (2.7 kb) and 2 kb of the 5' flanking region that contains the elements for anaerobic induction. A mutant lacking deactivase was shown to require exogenous electron acceptors for anaerobic growth with glucose. This revealed the identity of PFL-deactivase with the alcohol and acetaldehyde dehydrogenases of E. coli. The multienzyme represents a homopolymeric protein (approximately 40 x 96 kDa) requiring Fe2+ for all functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Dehydrogenase / genetics*
  • Alcohol Dehydrogenase / isolation & purification
  • Alcohol Dehydrogenase / metabolism
  • Aldehyde Oxidoreductases / genetics*
  • Aldehyde Oxidoreductases / isolation & purification
  • Aldehyde Oxidoreductases / metabolism
  • Anaerobiosis
  • Base Sequence
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli / growth & development
  • Escherichia coli Proteins
  • Genes, Bacterial*
  • Kinetics
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics*
  • Multienzyme Complexes / isolation & purification
  • Multienzyme Complexes / metabolism
  • Plasmids
  • Restriction Mapping

Substances

  • Escherichia coli Proteins
  • Multienzyme Complexes
  • Alcohol Dehydrogenase
  • adhE protein, E coli
  • Aldehyde Oxidoreductases
  • acetaldehyde dehydrogenase (acylating)

Associated data

  • GENBANK/X58797
  • GENBANK/X58798
  • GENBANK/X58799
  • GENBANK/X58800
  • GENBANK/X58801
  • GENBANK/X58802
  • GENBANK/X58803
  • GENBANK/X59501
  • GENBANK/X59502
  • GENBANK/X62841