Abstract
Loss of transforming growth factor-beta receptor III (TbetaRIII) correlates with loss of transforming growth factor-beta (TGF-beta) responsiveness and suggests a role for dysregulated TGF-beta signaling in clear cell renal cell carcinoma (ccRCC) progression and metastasis. Here we identify that for all stages of ccRCC TbetaRIII expression is downregulated in patient-matched tissue samples and cell lines. We find that this loss of expression is not due to methylation of the gene and we define GATA3 as the first transcriptional factor to positively regulate TbetaRIII expression in human cells. We localize GATA3's binding to a 10-bp region of the TbetaRIII proximal promoter. We demonstrate that GATA3 mRNA is downregulated in all stages, of ccRCC, mechanistically show that GATA3 is methylated in ccRCC patient tumor tissues as well as cell lines, and that inhibiting GATA3 expression in normal renal epithelial cells downregulates TbetaRIII mRNA and protein expression. These data support a sequential model whereby loss of GATA3 expression through epigenetic silencing decreases TbetaRIII expression during ccRCC progression.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / metabolism
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Blotting, Western
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Carcinoma, Renal Cell / genetics*
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Carcinoma, Renal Cell / metabolism
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Carcinoma, Renal Cell / pathology
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Cell Line, Tumor
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DNA Methylation
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Electrophoretic Mobility Shift Assay
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GATA3 Transcription Factor / genetics*
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GATA3 Transcription Factor / metabolism
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic*
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Gene Silencing*
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Humans
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Immunoenzyme Techniques
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Kidney Neoplasms / genetics*
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Kidney Neoplasms / metabolism
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Kidney Neoplasms / pathology
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Luciferases / metabolism
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Oligonucleotide Array Sequence Analysis
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Promoter Regions, Genetic
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Proteoglycans / genetics
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Proteoglycans / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, Transforming Growth Factor beta / genetics
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Receptors, Transforming Growth Factor beta / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Signal Transduction
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Transfection
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Transforming Growth Factor beta / genetics
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Transforming Growth Factor beta / metabolism
Substances
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Biomarkers, Tumor
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GATA3 Transcription Factor
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GATA3 protein, human
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Proteoglycans
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RNA, Messenger
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Receptors, Transforming Growth Factor beta
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Transforming Growth Factor beta
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betaglycan
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Luciferases