Inflammatory role of Toll-like receptors in human and murine adipose tissue

Mediators Inflamm. 2010:2010:823486. doi: 10.1155/2010/823486. Epub 2010 Mar 22.

Abstract

It was recently demonstrated that TLR4 activation via dietary lipids triggers inflammatory pathway and alters insulin responsiveness in the fat tissue during obesity. Here, we question whether other TLR family members could participate in the TLR-mediated inflammatory processes occurring in the obese adipose tissue. We thus studied the expression of TLR1, TLR2, TLR4, and TLR6 in adipose tissue. These receptors are expressed in omental and subcutaneous human fat tissue, the expression being higher in the omental tissue, independently of the metabolic status of the subject. We demonstrated a correlation of TLRs expression within and between each depot suggesting a coregulation. Murine 3T3-L1 preadipocyte cells stimulated with Pam3CSK4 induced the expression of some proinflammatory markers. Therefore, beside TLR4, other toll-like receptors are differentially expressed in human fat tissue, and functional in an adipocyte cell line, suggesting that they might participate omental adipose tissue-related inflammation that occurs in obesity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology
  • Adipocytes / drug effects
  • Adipocytes / metabolism
  • Adipose Tissue / metabolism*
  • Adult
  • Animals
  • Case-Control Studies
  • Cell Differentiation
  • Female
  • Gene Expression
  • Humans
  • Inflammation / etiology*
  • Inflammation / genetics
  • Inflammation / metabolism*
  • Inflammation Mediators / metabolism*
  • Lipopeptides / pharmacology
  • Mice
  • Middle Aged
  • Obesity / etiology
  • Obesity / genetics
  • Obesity / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Toll-Like Receptor 1 / genetics
  • Toll-Like Receptor 1 / metabolism
  • Toll-Like Receptor 2 / agonists
  • Toll-Like Receptor 2 / genetics
  • Toll-Like Receptor 2 / metabolism
  • Toll-Like Receptor 4 / genetics
  • Toll-Like Receptor 4 / metabolism
  • Toll-Like Receptor 6 / genetics
  • Toll-Like Receptor 6 / metabolism
  • Toll-Like Receptors / genetics
  • Toll-Like Receptors / metabolism*

Substances

  • Inflammation Mediators
  • Lipopeptides
  • Pam(3)CSK(4) peptide
  • RNA, Messenger
  • TLR2 protein, human
  • TLR4 protein, human
  • TLR6 protein, human
  • Tlr2 protein, mouse
  • Toll-Like Receptor 1
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptor 6
  • Toll-Like Receptors