Enhancer-binding activity of the tal-1 oncoprotein in association with the E47/E12 helix-loop-helix proteins

Mol Cell Biol. 1991 Jun;11(6):3037-42. doi: 10.1128/mcb.11.6.3037-3042.1991.

Abstract

Almost 30% of patients with T-cell acute lymphoblastic leukemia (T-ALL) bear structural alterations of tal-1, a presumptive proto-oncogene that encodes sequences homologous to the helix-loop-helix (HLH) DNA-binding and dimerization domain. Analysis of the tal-1 gene product reveals that its HLH domain mediates protein-protein interactions with either of the ubiquitously expressed HLH proteins E47 and E12. The resultant tal-1/E47 and tal-1/E12 heterodimers specifically recognize the E-box DNA sequence motif found in eucaryotic transcriptional enhancers. Hence, the tal-1 protein shares biochemical properties with other tissue-specific HLH proteins that control cell type determination during myogenesis (e.g., MyoD1) and neurogenesis (e.g., achaete-scute). The data suggest that HLH heterodimers involving tal-1 may function in vivo as transcriptional regulatory factors that influence cell type determination during hematopoietic development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Enhancer Elements, Genetic*
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Humans
  • Leukemia-Lymphoma, Adult T-Cell / genetics
  • Macromolecular Substances
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Protein Binding
  • Protein Biosynthesis
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogenes*
  • Recombinant Proteins / metabolism
  • T-Cell Acute Lymphocytic Leukemia Protein 1
  • TCF Transcription Factors
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factors*
  • Transcription, Genetic

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins
  • MAS1 protein, human
  • Macromolecular Substances
  • Oligonucleotide Probes
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins
  • Recombinant Proteins
  • T-Cell Acute Lymphocytic Leukemia Protein 1
  • TCF Transcription Factors
  • TCF7L1 protein, human
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factors
  • TAL1 protein, human
  • Glutathione Transferase