Abstract
It is not clear whether adult stem cell extracellular matrix (ECM) can regulate cancer cells. We demonstrated that the ECM produced by UCB-MSCs was able to arrest the growth of metastatic tumor cells by upregulating levels of PTEN in aggressive cancer cells. Human UCB-MSCs produced dickkopf (DKK1) are capable of inhibiting cancer cell proliferation but has no contribution to the tumor inhibition effect of UCB-MSC ECM. This study also provides an innovative approach to specifically examine the effect of stem cell microenvironments on cancer cells without the complexity of cell-cell interactions. In conclusion, human UCB-MSC ECM prohibits cancer cell proliferation.
2010 Elsevier Ireland Ltd. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adult
-
Animals
-
Blotting, Western
-
Breast Neoplasms / pathology*
-
Cell Culture Techniques / methods
-
Cell Division / drug effects
-
Cell Division / physiology*
-
Extracellular Matrix / physiology*
-
Female
-
Fetal Blood / cytology*
-
Green Fluorescent Proteins / genetics
-
Humans
-
Immunohistochemistry
-
Intercellular Signaling Peptides and Proteins / genetics
-
Mesenchymal Stem Cells / cytology*
-
Mitomycin / pharmacology
-
Neoplasm Metastasis / pathology
-
Neoplasm Metastasis / prevention & control*
-
PTEN Phosphohydrolase / genetics
-
RNA Interference
-
Reverse Transcriptase Polymerase Chain Reaction
Substances
-
DKK1 protein, human
-
Intercellular Signaling Peptides and Proteins
-
Green Fluorescent Proteins
-
Mitomycin
-
PTEN Phosphohydrolase
-
PTEN protein, human