Human umbilical cord blood mesenchymal stem cell-derived extracellular matrix prohibits metastatic cancer cell MDA-MB-231 proliferation

Cancer Lett. 2010 Oct 28;296(2):178-85. doi: 10.1016/j.canlet.2010.04.007.

Abstract

It is not clear whether adult stem cell extracellular matrix (ECM) can regulate cancer cells. We demonstrated that the ECM produced by UCB-MSCs was able to arrest the growth of metastatic tumor cells by upregulating levels of PTEN in aggressive cancer cells. Human UCB-MSCs produced dickkopf (DKK1) are capable of inhibiting cancer cell proliferation but has no contribution to the tumor inhibition effect of UCB-MSC ECM. This study also provides an innovative approach to specifically examine the effect of stem cell microenvironments on cancer cells without the complexity of cell-cell interactions. In conclusion, human UCB-MSC ECM prohibits cancer cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Blotting, Western
  • Breast Neoplasms / pathology*
  • Cell Culture Techniques / methods
  • Cell Division / drug effects
  • Cell Division / physiology*
  • Extracellular Matrix / physiology*
  • Female
  • Fetal Blood / cytology*
  • Green Fluorescent Proteins / genetics
  • Humans
  • Immunohistochemistry
  • Intercellular Signaling Peptides and Proteins / genetics
  • Mesenchymal Stem Cells / cytology*
  • Mitomycin / pharmacology
  • Neoplasm Metastasis / pathology
  • Neoplasm Metastasis / prevention & control*
  • PTEN Phosphohydrolase / genetics
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • DKK1 protein, human
  • Intercellular Signaling Peptides and Proteins
  • Green Fluorescent Proteins
  • Mitomycin
  • PTEN Phosphohydrolase
  • PTEN protein, human