Abstract
Prostate-specific membrane antigen (PSMA), an established enzyme-biomarker for prostate cancer, has attracted considerable attention as a target for imaging and therapeutic applications. We aimed to determine the effects of PSMA-targeted photodynamic therapy (PDT) on cytoskeletal networks in prostate cancer cells. PSMA-targeted PDT resulted in rapid disruption of microtubules (alpha-/beta-tubulin), microfilaments (actin), and intermediate filaments (cytokeratin 8/18) in the cytoplasm of LNCaP cells. The collapse of cytoplasmic microtubules and the later nuclear translocation of alpha-/beta-tubulin were the most dramatic alternation. It is likely that these early changes of cytoskeletal networks are partly involved in the initiation of cell death.
Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Actin Cytoskeleton / drug effects
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Actin Cytoskeleton / radiation effects
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Actins / metabolism
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Animals
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Antibodies, Monoclonal
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Biomarkers, Tumor / blood
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Biomarkers, Tumor / radiation effects
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Cell Death
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Cell Line, Tumor
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Cytoskeleton / pathology
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Humans
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Intermediate Filaments / drug effects
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Intermediate Filaments / radiation effects
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Keratins / immunology
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Male
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Mice
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Microtubules / drug effects
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Microtubules / radiation effects
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Photochemotherapy / methods*
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Prostate-Specific Antigen / blood
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Prostate-Specific Antigen / radiation effects*
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Prostatic Neoplasms / drug therapy
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Prostatic Neoplasms / genetics
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Prostatic Neoplasms / metabolism
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Prostatic Neoplasms / pathology
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Translocation, Genetic
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Tubulin / genetics
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Tubulin / immunology
Substances
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Actins
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Antibodies, Monoclonal
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Biomarkers, Tumor
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Tubulin
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Keratins
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Prostate-Specific Antigen