Lpin1 in human visceral and subcutaneous adipose tissue: similar levels but different associations with lipogenic and lipolytic genes

Am J Physiol Endocrinol Metab. 2010 Aug;299(2):E308-17. doi: 10.1152/ajpendo.00699.2009. Epub 2010 Jun 8.

Abstract

LPIN1 is a gene with important effects on lipidic and metabolic homeostasis. Human subcutaneous LPIN1 expression levels in adipose tissue are related with a better metabolic profile, including insulin sensitivity markers. However, there are few data on the regulation of LPIN1 in visceral adipose tissue (VAT). Our aim was to perform a cross-sectional analysis of VAT compared with subcutaneous (SAT) LPIN1 expression in a well-characterized obese cohort, its relation with the expression of genes involved in lipid metabolism, and the in vitro response to lipogenic and lipolytic stimuli. A downregulation of total LPIN1 mRNA expression in subjects with obesity was found in VAT similarly to that in SAT. Despite similar total LPIN1 mRNA levels in SAT and VAT, a close relationship with clinical parameters and with many lipogenic and lipolytic genes was observed primarily in SAT depot. As shown in the in vitro analysis, the low-grade proinflammatory environment and the insulin resistance associated with obesity may contribute to downregulate LPIN1 in adipose tissue, leading to a worse metabolic profile.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / drug effects
  • Adipocytes / metabolism
  • Adipose Tissue / cytology
  • Adipose Tissue / drug effects
  • Adipose Tissue / metabolism*
  • Adrenergic beta-Agonists / pharmacology
  • Adult
  • Aged
  • Blotting, Western
  • Body Mass Index
  • Cell Differentiation
  • Cells, Cultured
  • Cohort Studies
  • Cross-Sectional Studies
  • DNA, Complementary / biosynthesis
  • DNA, Complementary / genetics
  • Female
  • Gene Expression / genetics
  • Humans
  • Hypoglycemic Agents / pharmacology
  • Insulin / pharmacology
  • Isoproterenol / pharmacology
  • Lipid Metabolism / drug effects
  • Lipid Metabolism / genetics*
  • Lipolysis / drug effects
  • Lipolysis / genetics*
  • Male
  • Metabolic Syndrome / metabolism
  • Middle Aged
  • Nuclear Proteins / biosynthesis*
  • Nuclear Proteins / genetics
  • Phosphatidate Phosphatase
  • Subcutaneous Fat / cytology
  • Subcutaneous Fat / drug effects
  • Subcutaneous Fat / metabolism*
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Adrenergic beta-Agonists
  • DNA, Complementary
  • Hypoglycemic Agents
  • Insulin
  • Nuclear Proteins
  • Tumor Necrosis Factor-alpha
  • LPIN1 protein, human
  • Phosphatidate Phosphatase
  • Isoproterenol