Application of the nuclear factor-κB inhibitor pyrrolidine dithiocarbamate for the treatment of endometriosis: an in vitro study

Fertil Steril. 2010 Dec;94(7):2942-4. doi: 10.1016/j.fertnstert.2010.05.009. Epub 2010 Jun 19.

Abstract

This study demonstrated that pyrrolidine dithiocarbamate (PDTC), a potent nuclear factor-κB inhibitor, showed stronger inhibitory effects on nuclear factor-κB activation in endometriotic stromal cells than in normal endometrial stromal cells as determined by electrophoretic mobility shift assay and Western blot analysis. Pretreatment of endometriotic stromal cells with PDTC attenuated tumor necrosis factor-α-induced expressions of CD44s, matrix metalloproteinase-9, and vascular endothelial growth factor whereas reversed tumor necrosis factor-α-reduced expressions of tissue inhibitor of metalloproteinase-1 revealed by reverse transcriptase polymerase chain reaction and Western blot analysis, suggesting that PDTC may represent a novel therapeutic strategy for treatment of endometriosis.

MeSH terms

  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use
  • Case-Control Studies
  • Cells, Cultured
  • Drug Evaluation, Preclinical
  • Endometriosis / drug therapy*
  • Endometriosis / genetics
  • Endometriosis / metabolism
  • Endometriosis / pathology
  • Female
  • Gene Expression / drug effects
  • Humans
  • Hyaluronan Receptors / genetics
  • Hyaluronan Receptors / metabolism
  • Matrix Metalloproteinase 9 / genetics
  • Matrix Metalloproteinase 9 / metabolism
  • NF-kappa B / antagonists & inhibitors*
  • NF-kappa B / metabolism
  • Ovarian Cysts / pathology
  • Peritoneal Diseases / drug therapy*
  • Peritoneal Diseases / genetics
  • Peritoneal Diseases / metabolism
  • Peritoneal Diseases / pathology
  • Pyrrolidines / pharmacology
  • Pyrrolidines / therapeutic use*
  • Stromal Cells / drug effects
  • Stromal Cells / metabolism
  • Stromal Cells / pathology
  • Thiocarbamates / pharmacology
  • Thiocarbamates / therapeutic use*
  • Tissue Inhibitor of Metalloproteinase-1 / genetics
  • Tissue Inhibitor of Metalloproteinase-1 / metabolism

Substances

  • Antioxidants
  • CD44 protein, human
  • Hyaluronan Receptors
  • NF-kappa B
  • Pyrrolidines
  • Thiocarbamates
  • Tissue Inhibitor of Metalloproteinase-1
  • pyrrolidine dithiocarbamic acid
  • Matrix Metalloproteinase 9