Objective: To analyze a potential association of gene polymorphisms of the follicle-stimulating hormone receptor (FSHR) gene rs1394205 polymorphism and tyrosine hydroxylase (TH) rs2070762 polymorphism with pre-eclampsia.
Methods: The gene polymorphism of FSHR rs1394205 and TH rs2070762 were analyzed by real-time Quantitative polymerase chain reaction (TaqMan probe) in 105 patients with pre-eclampsia and 103 healthy pregnant subjects in Chinese Han population. Genotype and allele were assessed by direct counting methods. And the associations between 2 SNPs and pre-eclampsia were analyzed by chi-square test.
Results: The frequencies of the TH gene rs2070762 polymorphism CC, C/T and TT genotypes were 14.6%, 42.7% and 42.7% in pre-eclampsia group and 18.1%, 56.2% and 25.7% in normal control group respectively. The allelic frequency of rs2070762 polymorphism in TH in pre-eclampsia patients was significantly lower (P < 0.05) than that in normal pregnancies. There was a significant difference between two groups (P < 0.05). But there was no significant difference between mild and severe pre-eclampsia groups (P > 0.05). In contrast, no significant association was detected in the comparison of genotypes and allele of FSHR gene rs1394205 between pre-eclampsia patients and normal pregnancies.
Conclusion: The TH rs2070762 polymorphism was associated with the pathogenesis of pre-eclampsia. It suggests that the polymorphism in TH may be involved in the onset and development of pre-eclampsia.