Abstract
Deregulations of microRNA have been frequently observed in tongue squamous cell carcinoma (TSCC), but their roles in tumorigenesis are not entirely clear. Here, we reported the up-regulation of miR-24 in TSCC. MiR-24 up-regulation reduced the expression of RNA-binding protein dead end 1 (DND1). Knockdown of miR-24 led to enhanced expression of DND1. The direct targeting of miR-24 to the DND1 mRNA was predicted bioinformatically and confirmed by luciferase reporter gene assays. Furthermore, the miR-24-mediated change in DND1 expression suppressed the expression of cyclin-dependent kinase inhibitor 1B (CDKN1B), and also led to enhanced proliferation and reduced apoptosis in TSCC cells.
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Apoptosis / genetics
-
Base Sequence
-
Carcinoma, Squamous Cell / genetics
-
Carcinoma, Squamous Cell / metabolism*
-
Carcinoma, Squamous Cell / pathology
-
Cell Proliferation
-
Computational Biology
-
Cyclin-Dependent Kinase Inhibitor p27
-
Gene Expression Regulation, Neoplastic*
-
Humans
-
Intracellular Signaling Peptides and Proteins / antagonists & inhibitors
-
Intracellular Signaling Peptides and Proteins / genetics
-
MicroRNAs / metabolism*
-
Molecular Sequence Data
-
Neoplasm Proteins / antagonists & inhibitors*
-
Neoplasm Proteins / genetics
-
RNA, Messenger / metabolism
-
Sequence Analysis, RNA
-
Tongue Neoplasms / genetics
-
Tongue Neoplasms / metabolism*
-
Tongue Neoplasms / pathology
-
Up-Regulation
Substances
-
CDKN1B protein, human
-
Dnd1 protein, human
-
Intracellular Signaling Peptides and Proteins
-
MIRN24 microRNA, human
-
MicroRNAs
-
Neoplasm Proteins
-
RNA, Messenger
-
Cyclin-Dependent Kinase Inhibitor p27