Abstract
CCL20 is a chemokine that attracts immature dendritic cells. We show that monocytes, cells characteristic of the innate immune response, infected with Mycobacterium tuberculosis express the CCL20 gene at a much higher level than the same cells infected with non-tuberculous mycobacteria. Interferon (IFN)-γ, a fundamental cytokine in the immune response to tuberculosis, strongly inhibits both the transcription and the translation of CCL20. We have also confirmed that dendritic cells are a suitable host for mycobacteria proliferation, although CCL20 does not seem to influence their intracellular multiplication rate. The chemokine, however, down-regulates the characteristic production of reactive oxygen species (ROS) induced by M. tuberculosis in monocytes, which may affect the activity of the cells. Apoptosis mediated by the mycobacteria, possibly ROS-dependent, was also inhibited by CCL20.
© 2010 British Society for Immunology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies / immunology
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Antibodies / pharmacology
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Apoptosis / drug effects
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Apoptosis / immunology
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Chemokine CCL2 / immunology
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Chemokine CCL2 / metabolism
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Chemokine CCL20 / genetics
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Chemokine CCL20 / immunology
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Chemokine CCL20 / metabolism*
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Chemokine CCL20 / pharmacology
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Chemokines, CC / genetics
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Chemotaxis / drug effects
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Chemotaxis / immunology
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Colony Count, Microbial
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Culture Media, Conditioned / pharmacology
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Dendritic Cells / drug effects
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Dendritic Cells / immunology
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Dendritic Cells / microbiology
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Gene Expression / genetics
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Humans
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Interferon-gamma / pharmacology
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Legionella pneumophila / cytology
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Legionella pneumophila / immunology
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Macrophages / drug effects
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Macrophages / immunology
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Macrophages / microbiology
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Monocytes / drug effects
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Monocytes / immunology
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Monocytes / metabolism*
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Monocytes / microbiology*
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Mycobacterium avium / cytology
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Mycobacterium avium / immunology
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Mycobacterium kansasii / cytology
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Mycobacterium kansasii / immunology
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Mycobacterium tuberculosis / cytology
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Mycobacterium tuberculosis / immunology*
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Reactive Oxygen Species / metabolism*
Substances
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Antibodies
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CCL2 protein, human
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CCL20 protein, human
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Chemokine CCL2
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Chemokine CCL20
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Chemokines, CC
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Culture Media, Conditioned
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Reactive Oxygen Species
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Interferon-gamma