CCL20 is overexpressed in Mycobacterium tuberculosis-infected monocytes and inhibits the production of reactive oxygen species (ROS)

Clin Exp Immunol. 2010 Nov;162(2):289-97. doi: 10.1111/j.1365-2249.2010.04168.x. Epub 2010 Sep 1.

Abstract

CCL20 is a chemokine that attracts immature dendritic cells. We show that monocytes, cells characteristic of the innate immune response, infected with Mycobacterium tuberculosis express the CCL20 gene at a much higher level than the same cells infected with non-tuberculous mycobacteria. Interferon (IFN)-γ, a fundamental cytokine in the immune response to tuberculosis, strongly inhibits both the transcription and the translation of CCL20. We have also confirmed that dendritic cells are a suitable host for mycobacteria proliferation, although CCL20 does not seem to influence their intracellular multiplication rate. The chemokine, however, down-regulates the characteristic production of reactive oxygen species (ROS) induced by M. tuberculosis in monocytes, which may affect the activity of the cells. Apoptosis mediated by the mycobacteria, possibly ROS-dependent, was also inhibited by CCL20.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / immunology
  • Antibodies / pharmacology
  • Apoptosis / drug effects
  • Apoptosis / immunology
  • Chemokine CCL2 / immunology
  • Chemokine CCL2 / metabolism
  • Chemokine CCL20 / genetics
  • Chemokine CCL20 / immunology
  • Chemokine CCL20 / metabolism*
  • Chemokine CCL20 / pharmacology
  • Chemokines, CC / genetics
  • Chemotaxis / drug effects
  • Chemotaxis / immunology
  • Colony Count, Microbial
  • Culture Media, Conditioned / pharmacology
  • Dendritic Cells / drug effects
  • Dendritic Cells / immunology
  • Dendritic Cells / microbiology
  • Gene Expression / genetics
  • Humans
  • Interferon-gamma / pharmacology
  • Legionella pneumophila / cytology
  • Legionella pneumophila / immunology
  • Macrophages / drug effects
  • Macrophages / immunology
  • Macrophages / microbiology
  • Monocytes / drug effects
  • Monocytes / immunology
  • Monocytes / metabolism*
  • Monocytes / microbiology*
  • Mycobacterium avium / cytology
  • Mycobacterium avium / immunology
  • Mycobacterium kansasii / cytology
  • Mycobacterium kansasii / immunology
  • Mycobacterium tuberculosis / cytology
  • Mycobacterium tuberculosis / immunology*
  • Reactive Oxygen Species / metabolism*

Substances

  • Antibodies
  • CCL2 protein, human
  • CCL20 protein, human
  • Chemokine CCL2
  • Chemokine CCL20
  • Chemokines, CC
  • Culture Media, Conditioned
  • Reactive Oxygen Species
  • Interferon-gamma